- 中文名稱
NFkB (P50) ELISA Kit - KAA064
- 英文名字
- NFkB (P50) ELISA Kit - KAA064
- 供應(yīng)商
- Rockland
- 產(chǎn)品貨號
- KAA064
- 產(chǎn)品報價
- ￥詢價/1Kit
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
- 90%產(chǎn)品中國有現(xiàn)貨庫存。銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- The NF-kB/Rel family of transcription factors is comprised of several structurally related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), c-Rel/NF-kB [1]. Members of this family are responsible for regulating over 150 target genes, including the expression of inflammatory cytokines, chemokines, immunoreceptors and cell adhesion molecules. Because of this, NF-kB has often been called a ‘central mediator of the human immune response' [2]. Acting as dimers, these transcription factors bind to DNA sequences, collectively called kB, sites thereby regulating expression of target genes. In most cells, Rel/ NF-kB transcription complexes are present in an inactive form in the cytoplasm, bound to an inhibitor IkB. Certain stimuli result in the phosphorylation, ubiquitination and subsequent degradation of IkB proteins thereby enabling translocation of NF-kB into the nucleus [3]. The most common Rel/NF-kB dimer in mammals contains p50-RelA (p50/p65) heterodimers and is specifically called NF-kB. One of the target genes activated by NF-kB is that encoding IkBα. This feedback mechanism allows newly-synthesized IkBα to enter the nucleus, remove NF-kB from DNA and transport it back to the cytoplasm thereby restoring its inactive state. The importance of Rel/NF-kB transcription factors in human inflammation and certain diseases makes them attractive targets for potential therapeutics [4-6].
- 產(chǎn)品描述
- Rockland's NF-kB (p50) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kB response element is immobilized onto the bottom of wells of a 96 well plate (see Figure 1 on page 4). NF-kB contained in a nuclear extract specifically binds to the NF-kB response element. NF-kB (p50) is detected by addition of a specific primary antibody directed against NF-kB (p50). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. Rockland's NF-kB (p50) Transcription Factor Assay detects human NF-kB (p50). It will not cross-react with NF-kB (p65).
- 產(chǎn)品特點(diǎn)
- 該NFkB (P50) ELISA Kit - KAA064的詳細(xì)信息查看Rockland提供的產(chǎn)品說明書。
- 保存建議
- See kit insert for complete instructions.
- 其他
- Rockland成立于1962年，是一家老牌抗體原產(chǎn)企業(yè)，其位于美國費(fèi)城郊區(qū)的農(nóng)場保有近20種實(shí)驗(yàn)動物。Rockland公司為生命科學(xué)領(lǐng)域提供豐富的、高質(zhì)量的抗體，包括載脂蛋白、細(xì)胞周期/凋亡、細(xì)胞因子、細(xì)胞信號蛋白、酶類、細(xì)胞外基質(zhì)、轉(zhuǎn)錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應(yīng)用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產(chǎn)特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標(biāo)記二抗，應(yīng)用十分廣泛。
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- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請以廠家的英文說明書為準(zhǔn)。下單前，請瀏覽說明書確認(rèn)。
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