Immunoprecipitation Kit: DYKDDDDK (FLAG?) for immunoprecipitation and purification of recombinant proteins containing the FLAG? epitope tag - KBA-319-383-Immunoprecipitation Kit: DYKDDDDK (FLAG?) for immunoprecipitation and purification of recombinant proteins containing the FLAG? epitope tag - KBA-319-383代理/參數(shù)/廠家-免疫學試劑盒-艾美捷科技有限公司

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中文名稱: Immunoprecipitation Kit: DYKDDDDK (FLAG?) for immunoprecipitation and purification of recombinant proteins containing the FLAG? epitope tag - KBA-319-383

英文名字: Immunoprecipitation Kit: DYKDDDDK (FLAG?) for immunoprecipitation and purification of recombinant proteins containing the FLAG? epitope tag - KBA-319-383

供應商: Rockland

產(chǎn)品貨號: KBA-319-383

產(chǎn)品報價: ￥詢價/1Kit

產(chǎn)品說明書: 點擊查看

購買方式: 90%產(chǎn)品中國有現(xiàn)貨庫存。銀行轉賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868

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背景資料: Rockland Immunochemicals' Anti-DYKDDDDK (FLAG?) Kit for Immunoprecipitation allows for the purification by immunoprecipitation of recombinant proteins containing the FLAG? epitope tag provided by the user. The kit relies upon the high specificity of monoclonal antibody raised against the FLAG? epitope tag. This method is far easier and less costly than using antibodies produced against the recombinant protein itself therefore saving time and resources. Using the agarose bound antibody in this kit allows for efficient binding of FLAG? tag proteins without the need for preliminary steps or calibration. The immunoprecipitated FLAG? tag protein can be efficiently eluted from the agarose beads using a low pH elution step. The user is able to further characterize the resultant purified protein by size, post-translational modification, western blot and other assays.

產(chǎn)品描述: Rockland Immunochemicals' Anti-DYKDDDDK (FLAG?) Kit for Immunoprecipitation is intended to provide a simple, reliable and convenient purification system for recombinant proteins containing the FLAG? epitope tag. Immunoprecipitation is a powerful technique for the isolation of proteins or protein complexes. Immunoprecipitation consists of several steps including cell lysis, binding of specific antigen to an antibody, antibody-antigen complex precipitation, precipitant wash steps and the dissociation of antigen from the complex. The FLAG? epitope tag is a small but highly immunogenic peptide DYKDDDDK (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C), which allows fusion proteins to retain their original conformation and function. The hydrophilic character of FLAG? increases the likelihood that it will be located on the surface of the fusion protein where it is accessible to antibodies. Rockland Immunochemicals' Anti-DYKDDDDK (FLAG?) Kit for Immunoprecipitation allows a rapid and efficient immunoprecipitation and elution of an active FLAG? -tagged recombinant protein in less than 2 hours. The immunoprecipitation is performed with anti-FLAG? antibody coupled to agarose beads, which are generated by covalently linking agarose to a highly specific mouse monoclonal antibody raised against FLAG?. The provided protocol is a guideline. Any procedure can be altered according to specific experimental requirements. This kit is sufficient to perform 50 X 20 μL reactions and is stable for at least 1 year when stored as indicated.

產(chǎn)品特點: 該Immunoprecipitation Kit: DYKDDDDK (FLAG?) for immunoprecipitation and purification of recombinant proteins containing the FLAG? epitope tag - KBA-319-383的詳細信息查看Rockland提供的產(chǎn)品說明書。

保存建議: See kit insert for complete instructions.

其他: Rockland成立于1962年，是一家老牌抗體原產(chǎn)企業(yè)，其位于美國費城郊區(qū)的農場保有近20種實驗動物。Rockland公司為生命科學領域提供豐富的、高質量的抗體，包括載脂蛋白、細胞周期/凋亡、細胞因子、細胞信號蛋白、酶類、細胞外基質、轉錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產(chǎn)特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標記二抗，應用十分廣泛。