The DyLight? multiplex 649/549/800 Trio Fluorescent Western blot kit is suited for simultaneous detection and quantification of specific protein populations in a biological sample. Using a combination of three antibodies selected for minimal cross reactivity, fluorescent detection method enables simultaneous quantitative analysis of multiple proteins within the same sample on the same blot. The DyLight? multiplex 649/549/800 Trio Western blot kit contain all the necessary components that are optimized for the simultaneous detection of multiple proteins on the same blot using DyLight?-dye labeled secondary antibodies that are visualized in different fluorescence channels (649/549/800). The kit also includes blocking buffer, wash buffer, pre-stained protein standard and an incubation box for convinience and ultimate performance with minimal or no optimization. The fluorescent dyes such as DyLights? when conjugated to secondary antibodies, offer a variety of benefits over traditional detection methods such as colorimetric and chemiluminescent detection. Multiplex detection using the correct lighting and filter conditions, enables the quantitation of multiple proteins and eliminates the need to strip and reprobe. Other benefits of fluorescent Western blotting include increased sensitivity, excellent signal stability over time as well as precise quantitative analysis with broader dynamic range and high linearity. Due to their exceptional photostability, DyLight? dye conjugates can be archived and visualized several times without a decrease in signal.
產(chǎn)品描述
This DyLight? Multiplex 649/549/800 Trio Western Blot Kit contains: Goat anti-Mouse IgG (H+L) DyLight? 649; Goat anti-Chicken IgG (H+L) DyLight? 549; Goat anti-Rabbit IgG (H+L) DyLight? 800; Opal pre-stained protein standard; Wash buffer (10x TTBS); Blocking buffer (2x) and incubation box. This kit is suitable for fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial gel imaging systems.
產(chǎn)品特點
該DyLight? Multiplex 649/549/800 Trio Western Blot Kit - KFA018的詳細信息查看Rockland提供的產(chǎn)品說明書。
保存建議
The secondary antibodies should be stored at 4°C prior to reconstitution. For extended storage aliquot contents and freeze at -20°C. Avoid freeze/thaw cycles. The wash buffer and blocking buffer can be stored at 2-8°C prior to opening. The pre-stained protein Western standards should be stored at 2-8°C. The pre-stained protein standards can also be stored at room temperature for up to 6 months or at -20°C for up to two years.