【干冰】CRISPR in a Box? - Starter Kit - KGA-0100-【干冰】CRISPR in a Box? - Starter Kit - KGA-0100代理/參數(shù)/廠家-免疫學(xué)試劑盒-艾美捷科技有限公司

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背景資料: The CRISPR in a Box? - Starter Kit (KGA-0100) utilizes revolutionary CRISPR-Cas technology. The model system provided uses CRISPR-Cas12a ribonucleoprotein (RNP) in vitro to modify a segment within the lacZ gene using a single-stranded donor DNA template and a mammalian cell-free extract. The single-stranded donor DNA template contains flanking arms of homology to the target site and directs the integration of a NotI restriction enzyme site. The products of this reaction are transformed into Escherichia coli (E. coli) bacteria and a visual, phenotypic readout of a blue to white color change is seen. Additional downstream sequencing or restriction enzyme digestion can be used to confirm presence of the integrated NotI site.
One of the many advantages of the in vitro system is the ability to analyze all gene editing products from a single reaction. Other major advantages of utilizing this system include, but are not limited to, exploring different repair pathways (HDR, NHEJ, etc.), targeting a variety of unique genes, and evaluating the efficiency of diverse Cas proteins.

產(chǎn)品描述: The CRISPR in a Box?- Starter Kit is an easy-to-use kit that contains the latest in gene editing technology. This technology was based on the pioneering discoveries of the Gene Editing Institute, which are currently being used to explore next-generation medical therapies for diseases like cancer and sickle-cell anemia.

產(chǎn)品特點(diǎn): 該【干冰】CRISPR in a Box? - Starter Kit - KGA-0100的詳細(xì)信息查看Rockland提供的產(chǎn)品說(shuō)明書。

保存建議: Store all kit components at -20°C for up to 12 months or -80°C for long-term storage.

其他: Rockland成立于1962年，是一家老牌抗體原產(chǎn)企業(yè)，其位于美國(guó)費(fèi)城郊區(qū)的農(nóng)場(chǎng)保有近20種實(shí)驗(yàn)動(dòng)物。Rockland公司為生命科學(xué)領(lǐng)域提供豐富的、高質(zhì)量的抗體，包括載脂蛋白、細(xì)胞周期/凋亡、細(xì)胞因子、細(xì)胞信號(hào)蛋白、酶類、細(xì)胞外基質(zhì)、轉(zhuǎn)錄因子(NFkB)和泛素（Ubiquitin）等識(shí)別抗體，應(yīng)用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產(chǎn)特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標(biāo)記二抗，應(yīng)用十分廣泛。