CellCountEZ? Cell Survival Assay Kit - KLD-001-CellCountEZ? Cell Survival Assay Kit - KLD-001代理/參數(shù)/廠家-免疫學(xué)試劑盒-艾美捷科技有限公司

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背景資料: CellCountEZ? Cell Survival Assay Kit is a tissue culture media-based assay that can measure metabolically active live cells and quantify cell death caused by radiation, chemotherapeutics or toxins. CellCountEZ? is based on the ability of mammalian cells to rapidly and efficiently convert hydroxyethyl disulfide (HEDS) into mercaptoethanol (ME) through a bioreduction mechanism. Bioconversion of HEDS to ME relies on the activity of the oxidative pentose phosphate cycle (OPPC). CellCountEZ? measures ME in the extracellular medium without the need for cellular lysis and extraction methods, this preserves the ability to perform other cellular tests in the same culture system. CellCountEZ? is readily soluble, membrane permeable and converted by live cells intracellularly before transport into the extracellular culture media. CellCountEZ? offers many advantages that make it superior to common existing methods for quantifying cell growth and survival.

產(chǎn)品描述: CellCountEZ? Cell Survival Assay Kit contains enough reagents for 1000 assays using a 96-well plate. This assay gives a linear response for cells (0; 1,000; 5,000; 10,000; 20,000; 40,000) plated in 100 μl growth medium with up to 15% fetal bovine serum in a 96-well plate and measured 20 hours after plating. Drug toxicity could be measured for up to 4 days after treatment with drugs using this assay for 5,000 cells plated in 100 μl growth medium with up to 15% fetal bovine serum in a 96-well plate.

產(chǎn)品特點(diǎn): 該CellCountEZ? Cell Survival Assay Kit - KLD-001的詳細(xì)信息查看Rockland提供的產(chǎn)品說明書。

保存建議: Store kit at 2-8° C prior to opening. See kit insert for complete instructions.

其他: Rockland成立于1962年，是一家老牌抗體原產(chǎn)企業(yè)，其位于美國費(fèi)城郊區(qū)的農(nóng)場保有近20種實(shí)驗(yàn)動物。Rockland公司為生命科學(xué)領(lǐng)域提供豐富的、高質(zhì)量的抗體，包括載脂蛋白、細(xì)胞周期/凋亡、細(xì)胞因子、細(xì)胞信號蛋白、酶類、細(xì)胞外基質(zhì)、轉(zhuǎn)錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應(yīng)用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產(chǎn)特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標(biāo)記二抗，應(yīng)用十分廣泛。