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中文名稱

Rat IGF-1 AccuSignal ELISA Kit - KOA0194

英文名字
Rat IGF-1 AccuSignal ELISA Kit - KOA0194
供應(yīng)商
Rockland
產(chǎn)品貨號(hào)
KOA0194
產(chǎn)品報(bào)價(jià)
¥詢價(jià)/1Kit
產(chǎn)品說明書
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產(chǎn)品新聞
背景資料
Insulin-like growth factor 1(IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD. The IGF1 gene, mapped on 12q22-q24.1, contains 5 exons. Exons 1-4 encode the 195-amino acid precursor(IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide(IGF1A). The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98% of IGF-1 is always bound to one of 6 binding proteins(IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.
產(chǎn)品描述
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml rat IGF-1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each rat IGF-1 standard solution and each sample be measured in duplicate.
產(chǎn)品特點(diǎn)
該Rat IGF-1 AccuSignal ELISA Kit - KOA0194的詳細(xì)信息查看Rockland提供的產(chǎn)品說明書。
保存建議
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
其他
Rockland成立于1962年,是一家老牌抗體原產(chǎn)企業(yè),其位于美國費(fèi)城郊區(qū)的農(nóng)場保有近20種實(shí)驗(yàn)動(dòng)物。Rockland公司為生命科學(xué)領(lǐng)域提供豐富的、高質(zhì)量的抗體,包括載脂蛋白、細(xì)胞周期/凋亡、細(xì)胞因子、細(xì)胞信號(hào)蛋白、酶類、細(xì)胞外基質(zhì)、轉(zhuǎn)錄因子(NFkB)和泛素(Ubiquitin)等識(shí)別抗體,應(yīng)用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外,Rockland也生產(chǎn)特別齊全的二抗,包括純化、生物素、HRP/ALP、熒光標(biāo)記二抗,應(yīng)用十分廣泛。
注意
該頁面的中文產(chǎn)品信息的翻譯,僅供參考。準(zhǔn)確的產(chǎn)品信息請以廠家的英文說明書為準(zhǔn)。下單前,請瀏覽說明書確認(rèn)。
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