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中文名稱(chēng)

【干冰】Small Sample Targeted ChIP Kit - KOA0886

英文名字
【干冰】Small Sample Targeted ChIP Kit - KOA0886
供應(yīng)商
Rockland
產(chǎn)品貨號(hào)
KOA0886
產(chǎn)品報(bào)價(jià)
￥詢(xún)價(jià)/1Kit
產(chǎn)品說(shuō)明書(shū)
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產(chǎn)品新聞
背景資料
Association between proteins and DNA is a major mechanism in many vital cellular functions such as gene transcription and epigenetic silencing. It is crucial to understand these interactions and the mechanisms by which they control and guide gene regulation pathways and cellular proliferation. Chromatin immunoprecipitation (ChIP) is a technique to analyse the association of proteins with specific genomic regions in intact cells. ChIP can be used to study changes in epigenetic signatures, chromatin remodelling and transcription regulator recruitment to specific genomic sites. In ChIP, living cells are first fixed with a reversible crosslinking agent to stabilize protein-DNA interactions. The most widely used reagent to fix cells is formaldehyde which generates covalent bonds between amino or imino groups of proteins and nucleic acids. Formaldehyde treatment crosslinks both DNA-protein as well as protein-protein complexes. However conventional ChIP protocols require high numbers of cells (hundreds of thousands cells at least) limiting the application for ChIP technology to few cell samples. More recently, ChIP assays on smallest amount of cells have been reported. Nevertheless the procedure requires tedious optimization of several reaction conditions to face the increased background observed in ChIP performed with reduced amount of cells. That might consequently lead to considerable time and lab expenditures. To reduce these tedious steps, Small Sample Targeted ChIP Kit has optimized reagents and protocol to enable successful ChIP on as few as 10 000 cells. Moreover, the Small Sample Targeted ChIP Kit protocol has been thoroughly optimised for ChIP followed by high-throughput sequencing on Illumina? Next-Gen sequencers.
產(chǎn)品描述
The different steps of the ChIP assay are cell fixation (crosslinking), chromatin shearing, immunoprecipitation, reverse crosslinking followed by DNA purification and analysis of the immunoprecipitated DNA. Suitable for 10 reactions.
產(chǎn)品特點(diǎn)
該【干冰】Small Sample Targeted ChIP Kit - KOA0886的詳細(xì)信息查看Rockland提供的產(chǎn)品說(shuō)明書(shū)。
保存建議
See kit insert for components stored at 2-8°C and -20°C. Do not freeze Protein A and magnetic beads.
其他
Rockland成立于1962年，是一家老牌抗體原產(chǎn)企業(yè)，其位于美國(guó)費(fèi)城郊區(qū)的農(nóng)場(chǎng)保有近20種實(shí)驗(yàn)動(dòng)物。Rockland公司為生命科學(xué)領(lǐng)域提供豐富的、高質(zhì)量的抗體，包括載脂蛋白、細(xì)胞周期/凋亡、細(xì)胞因子、細(xì)胞信號(hào)蛋白、酶類(lèi)、細(xì)胞外基質(zhì)、轉(zhuǎn)錄因子(NFkB)和泛素（Ubiquitin）等識(shí)別抗體，應(yīng)用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產(chǎn)特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標(biāo)記二抗，應(yīng)用十分廣泛。
注意
該頁(yè)面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說(shuō)明書(shū)為準(zhǔn)。下單前，請(qǐng)瀏覽說(shuō)明書(shū)確認(rèn)。
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