Leukotriene C4 (LTC4) BioAssay ELISA Kit-Leukotriene C4 (LTC4) BioAssay ELISA Kit代理/參數(shù)/廠家-細(xì)胞分析試劑盒-艾美捷科技有限公司

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中文名稱: Leukotriene C4 (LTC4) BioAssay ELISA Kit

英文名字: Leukotriene C4 (LTC4) BioAssay ELISA Kit

供應(yīng)商: USBiological

產(chǎn)品貨號(hào): L2047-10

產(chǎn)品報(bào)價(jià): ￥1/1Kit

產(chǎn)品說(shuō)明書(shū): 點(diǎn)擊查看

購(gòu)買方式: 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868

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產(chǎn)品描述: Leukotriene C4 (LTC4) is a potent mediator of immediate hypersensitivity, bronchoconstriction, smooth muscle contraction, increased vascular permeability and epithelial mucous secretion. It is derived from arachidonic acid through the 5-lipoxygenase pathway. Arachidonic acid is first converted to 5-hydroperoxy derivative which is then transformed to Leukotriene A4 (LTA4). Enzymatic addition of glutathione to LTA4 produces LTC4.Principle of the Assay:This is an ELISA (Enzyme-Linked Immunosorbent Assay) for the quantitative analysis of Leukotriene C4 levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the Leukotriene C4 in the sample for a limited number of binding sites. First, the sample or standard solution is added to the microplate. Next, the diluted enzyme conjugate is added and the mixture is shaken and incubated at room temperature for one hour. During the incubation, competition for binding sites is taking place. The plate is then washed, removing all the unbound material. The bound enzyme conjugate is detected by the addition of substrate, which generates an optimal color after 30 minutes. Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650nm. The extent of color development is inversely proportional to the amount of Leukotriene C4 in the sample or standard. For example, the absence of Leukotriene C4 in the sample will result in a bright blue color, whereas the presence of Leukotriene C4 will result in decreased or no color development.Kit Components:L2047-10A: ELISA Buffer: 1x30ml. To be used to dilute enzyme conjugate and Leukotriene C4 standardsL2047-10B: Wash Buffer 10X: 1x20ml. To be diluted 10X with deionized water. This is used to wash all unbound enzyme conjugate, samples and standards from the plate after the one hour incubation.L2047-10C: Substrate: 1x20ml. Stabilized 3,3', 5,5' Tetramethylbenzidine (TMB) plus hydrogen peroxide (H2O2) in a single bottle. It is used to develop the color in the wells after they have been washed.L2047-10D: Extraction Buffer 5X: 1x30ml. To be diluted 5X with deionized water. This is used for diluting extracted and non-extracted samples..*L2047-10E: Leukotriene C4 (HRP): 2x75ul. Lyophilized LTC4 HRP conjugate. Reconstitute one vial with 75ul of deionized water for a 50X stock solution.L2047-10F: Leukotriene Standard C4: 1x100ul Leukotriene C4 standard at the concentration of 1ug/ml.L2047-10G: Leukotriene C4 Microtiter Plate: 1x96 well MaxiSorp Nunc microplate with anti-LTC4 rabbit antibody precoated on each well. The plate is ready to use as is. Do not wash.Storage and Stability:Store *L2047-10E powder at -20°C liquid at 4°C. Do not freeze. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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保存建議: 建議收到Leukotriene C4 (LTC4) BioAssay ELISA Kit產(chǎn)品后將其置于4°C/-20°C保存。

其他: Usbiological公司是美國(guó)著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過(guò)50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。