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細(xì)胞分析試劑盒
中文名稱

Luteinizing Hormone, Human (LH) BioAssay ELISA Kit

英文名字
Luteinizing Hormone, Human (LH) BioAssay ELISA Kit
供應(yīng)商
USBiological
產(chǎn)品貨號(hào)
L7500-01
產(chǎn)品報(bào)價(jià)
¥1/96Tests
產(chǎn)品說明書
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產(chǎn)品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國(guó)區(qū)域總代理,是行業(yè)中少有的致力于服務(wù)客戶,幫助客戶,且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營(yíng)銷團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng),專業(yè)的產(chǎn)品和解決方案支持,穩(wěn)健快捷的交貨周期,優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù),在意您的成功!
產(chǎn)品描述
hLH (human Luteinizing Hormone (hLH) is found in the anterior pituitary gland. It induces ovulation and regulates progesterone and estrogen production by the ovaries and testes. LH levels are measured to aid diagnosing the source of certain infertility problems and to ascertain the existence of a pituitary tumor. Intended Use:This human LH ELISA kit is to be used for the in vitro quantitative determination of human luteinizing hormone (LH) concentrations in serum.Principles of the Assay: This LH enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre- coated with a monoclonal antibody specific for LH. Standards or samples are then added to the microtiter plate wells and LH if present, will bind to the antibody pre-coated on the wells. In order to quantitate the amount of LH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for LH are added to each well to “sandwich” the LH immobilized on the plate. The microtiter plate undergoes an incubation, then the wells are thoroughly washed to remove all unbound components. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LH and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm. In order to measure the concentration of LH in the sample, this Human LH ELISA Kit includes a set of calibration standards (6 standards). The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density (O.D.) versus LH concentration (mIU/ml). The concentration of LH in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sensitivity: <3mIU/mlRange: 3-240mIU/mlKit Components:L7500-01A: Microtiter Plate 1x96 wellsL7500-01B: LH (HRP) 1x12mlL7500-01C: Standard (240 mIU) 1x vialL7500-01D: Standard (120 mIU) 1x1vialL7500-01E: Standard (60 mIU) 1x1vialL7500-01F: Standard (30 mIU) 1x1vialL7500-01G: Standard (7.5 mIU) 1x1vialL7500-01H: Standard (0 mIU) 1x1vialL7500-01J:. Substrate A 1x10mlL7500-01K: Substrate B 1x10mlL7500-01L: Stop Solution 1x7mlStorage and Stability:Store at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
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保存建議
建議收到Luteinizing Hormone, Human (LH) BioAssay ELISA Kit產(chǎn)品后將其置于4°C保存。
其他
Usbiological公司是美國(guó)著名的抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品,為科研用戶提供了諸多超值選擇。
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