Not Suitable for WB; IHC, Frozen; IF, Frozen; IC; FC; Immunohistochemistry, immunofluorescence, flow cytometry, NGF receptor p75 dynamics, retrograde transport studies, study of intracellular trafficking. Suggested working dilutions: For immunohistochemistry a concentration of 1-2 μg/mL is recommended. The antibody is not appropriate for Western Blots. The recommended concentration for FACS is 20 μg/mL and at least 1 in 5000 dilution is recommended for 1-site ELISA. Optimal working dilution should be determined by the end user. MC192 is not suitable as a blocking agent, although it has been incorrectly used for this purpose in many published works. The antibody was generated specifically by screening for monoclonals that had the ability to ENHANCE the binding of NGF, the natural ligand for p75. Therefore, this antibody is particularly unusual. The full details can be found in the original paper, which is listed on our datasheet (see Chandler et al, 1984). Biosensis recommends optimal dilutions/concentrations should be determined by the end user. The FITC version of MC192 is primarily targeted for FACS or IF applications on live or lightly fixed cells. Antibody will not work in traditional formalin fixed tissues.
免疫原
Rat p75NTR
來源宿主
小鼠
反應(yīng)性
反應(yīng)種屬:大鼠. 不反應(yīng)的種屬: 小鼠 or 人 p75 NGFR
保存建議
廠家建議冰袋運輸。該抗體偶聯(lián)物應(yīng)保存于4 °C ,添加適當(dāng)?shù)目股兀行谧罡邽?個月。