IH(P), Paraffin embedded; IC; FC: Flow Cytometry: Unfixed cells preferred, acetone fixed or quickly fixed 1% PLP fixed cells can be used. IH: Immunohistochemical studies of rat fresh frozen tissue sections and paraffin-embedded tissue sections following either periodate-lysine-paraformaldehyde (PLP) fixation, or acetone. Works on very lightly PFA fixed, frozen tissues. (perfusion only 4% PFA 10-15' no post-fix). Epitope can be sensitive to fixation. Dilutions detection method dependent 1:100 to 1:200 recommended. IC: Unfixed preferred, or acetone fixed cells; 5-10', 2% PLP fixed cells, 1-2μg/mL. Dilution is detection method dependent. Immunoprecipitation: use rabbit anti-mouse or anti-mouse IgG beads for capture only. The use of protein A or protein G is not recommended. 1-5μg/mL in restricted volumes. Clone does not work in traditional reduced westerns. Use immunoprecipitation to resolve reactive protein bands. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
Rat peritoneal macrophages, whole cells. (Robinson, AP et al Immunology 1986 57 239-247)
來(lái)源宿主
小鼠
反應(yīng)性
Mis-information exists concerning reactivity to 小鼠, 人 CD11b/c with OX-42 from various vendors. Biosensis has not verified that OX42 反應(yīng)種屬:小鼠, 人,, ONLY recommends the clone only for 大鼠 as the original paper, most papers use the OX family of clones on 大鼠.