Western blotting: 0.5-2 μg/mL , SDS-PAGE on Bis-Tris gel 4-12%, 5% beta-mercaptoethanol, primary antibody O/N incubation in 5% skim milk/TBST. Secondary is anti-mouse-HRP, 1/6000 dilution, 2h at room temperature. Blot developed on Li-Cor? C-DiGit? lot Scanner.
IHC: Frozen or PEG embedded tissues tested (PEG embedding, see Klosen P et al (1993) J Histochem Cytochem. 41(3):455-63). Conditions tested: 1-10 μg/mL in PBS, 48 hours, followed by detection via directly conjugated fluorescent anti-mouse secondary. Antibody not yet tested on paraffin embedded sections. Other immunohistochemistry methods not yet tested but are expected to be reactive.
ICC: 4% formaldehyde fixed cells tested; requires permeabilization step as antigen epitope is intracellular. Suggested primary antibody concentration: 1-2 μg/mL.
Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (C-GSLKPEDAEVFKDSMVPGEK) as a part of the C-terminal rat VR1 protein (aa: 819-838) has been used as the immunogen.
來(lái)源宿主
小鼠
反應(yīng)性
This antibody clone is known to react with 大鼠, 小鼠 TrpV1. predicted to react with 豚鼠 due to sequence homology.