Mir-10b (MicroRNA 10b) Detection Kit, BioAssay-Mir-10b (MicroRNA 10b) Detection Kit, BioAssay代理/參數(shù)/廠家-細(xì)胞分析試劑盒-艾美捷科技有限公司

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中文名稱: Mir-10b (MicroRNA 10b) Detection Kit, BioAssay

英文名字: Mir-10b (MicroRNA 10b) Detection Kit, BioAssay

供應(yīng)商: USBiological

產(chǎn)品貨號(hào): M3949-97

產(chǎn)品報(bào)價(jià): ￥1/1Kit

產(chǎn)品說(shuō)明書(shū): 點(diǎn)擊查看

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背景資料: USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國(guó)區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營(yíng)銷團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！

產(chǎn)品描述: Introduction:MicroRNAs (miRNAs) are endogenous regulators of gene expression that are encoded in the genomes of plants, animals and viruses, then processed into ~19-23 nt, single-stranded molecules that become incorporated into the RNA-induced silencing complex (RISC). RISC mediates down-regulation of gene expression through translational inhibition, transcript cleavage, or both. There is emerging evidence that some microRNAs can function as oncogenes or tumor suppressors. Recent study showed that microRNA-10b (miR-10b) is highly expressed in metastatic breast cancer cells and positively regulates cell migration and invasion. Designed for use with MicroRNA qRT-PCR primer sets, the miR-10b One-Step qRT-PCR Detection Kit provides a one-step, simple, robust, inexpensive assay for quantitative analysis of mir-10b expression from total RNA samples, or small RNAs enriched samples. MicroRNA qRT-PCR primer sets were uniquely designed and optimized for detecting mature miRNA with high specificity and sensitivity at low PCR annealing temperature. The reverse PCR primer is also served as RT primer for reverse transcription. This kit contains an enzyme mix, a 2X-concentrated qRT-PCR reaction mixture and a miRNA primer set that contain all the reagents (except templates) needed for running qRT-PCR assay. The passive reference dye ROX is included in a separate tube to make this kit adaptable for many real-time QPCR platforms. Total RNA from metastatic breast cancer cell line MDA-MB-231 is supplied with the kit as positive control. Features:? Convenient - All reaction components are supplied for quick and easy set up ? Save time – One-step qRT-PCR procedure reduces setup time and liquid handling steps ? Wide dynamic range: good linearity and excellent PCR efficiency over a 6 logs of dynamic range ? High Sensitivity - detect miRNA expression in as low as 2.5pg total RNA. ? Flexible – Compatible with most of the real-time PCR instruments.Applications:? Mir-10b detection and quantification ? Breast cancer metastasis studiesDescription: Components in this kit are prepared with pure chemicals according to our proprietary technology. Mir-10b One-Step qRT-PCR Detection Kit provides a one-step, simple, robust, inexpensive assay for detection and quantitative analysis of mir-10b expression from total RNA samples, or small RNAs enriched samples. Quality Control 1 kit of this lot has been tested for amplifying miR-10b from total RNA of metastatic breast cancer cell line MDA-MB-231 over a 6 logs of dynamic range using Stratagene's Mx3005P as a real time PCR instrument. Good linearity and great PCR efficiency is observed and consistent with the previous lot. Components: Reagents are sufficient for 200 assays.Item Amount1. MicroRNA 2X qRT-PCR Reaction Mixture, 1.25ml x 22. MicroRNA qRT-PCR Enzyme Mix*, 100ul3. ROX Reference Dye, 100ul4. miR-10b qRT-PCR Primer Set (25X) 200ul5. MDA-MB-231 Total RNA (25ng/ul) 100ul6. Nuclease-Free PCR Grade Water 3ml* microRNA qRT-PCR enzyme mix contains Reverse Transcriptase, RNase Inhibitor, and Hotstart Taq DNA Polymerase. Storage and Stability Upon receipt, store all components at -20°C in a constant temperature freezer. Avoid repeated freeze/thaw cycles. When stored under these conditions this kit is stable for one year after ship date. The dye in MicroRNA 2x qRT-PCR Mixture and the ROX reference dye are light sensitive and should be kept away from light whenever possible.

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保存建議: 建議收到Mir-10b (MicroRNA 10b) Detection Kit, BioAssay產(chǎn)品后將其置于-20°C保存。

其他: Usbiological公司是美國(guó)著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過(guò)50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。