Mucin 16, Human (MUC16, MUC-16, CA125, CA-125, Cancer Antigen 125, FLJ14303, Mucin 16 Cell Surface Associated, Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125) BioAssay ELISA Kit-Mucin 16, Human (MUC16, MUC-16, CA125, CA-125, Cancer Antigen 125, FLJ14303, Mucin 16 Cell Surface Associated, Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125) BioAssay ELISA Kit代理/參數/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: Mucin 16, Human (MUC16, MUC-16, CA125, CA-125, Cancer Antigen 125, FLJ14303, Mucin 16 Cell Surface Associated, Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125) BioAssay ELISA Kit

英文名字: Mucin 16, Human (MUC16, MUC-16, CA125, CA-125, Cancer Antigen 125, FLJ14303, Mucin 16 Cell Surface Associated, Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125) BioAssay ELISA Kit

供應商: USBiological

產品貨號: M4701-94H

產品報價: ￥1/96Tests

產品說明書: 點擊查看

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產品描述: Sample Type:SerumIntended Use:For the quantitative determination of the Cancer Antigen CA125 concentration in human serum.Introduction:Cancer Antigen 125 (CA125) is a surface antigen associated with epithelial ovarian cancer. In serum, CA125 is associated with a high molecular weight glycoprotein. Published studies have indicated that elevated serum CA125 levels can be found in individuals with serious endometroid, clear-cell and undifferentiated ovarian carcinoma. The serum CA125 concentration is greater than 35 units per ml in 60% of women with ovarian cancer and > 80% of patients with disseminated ovarian cancer. The serum CA125 is elevated in 1% of normal healthy women, 3% of normal healthy women with benign ovarian diseases, 6% of patients with non-neoplastic conditions (including but not limited to first trimester pregnancy, menstruation, endometriosis, uterine fibrosis, acute salphingitis, hepatic diseases and inflammation of peritoneum, pericardium or pleura). Serial determinations of serum CA125 as well as pelvic examination increase the test specificity. Serum CA125 concentration may be useful in monitoring treatment and distinguishing between good response to treatment and progressive malignant disease with poor therapeutic response. To date, CA125 is the most sensitive marker for residual epithelial ovarian cancer. CA125 may also be elevated in patients with lung, cervical, fallopian tube, and uterine cancer and endometriosis.Test Principle:The CA125 ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes a monoclonal antibody directed against a distinct antigenic determinant on the intact CA125 molecule is used for solid phase immobilization (on the microtiter wells). A rabbit anti-CA125 antibody conjugated to horseradish peroxidase (HRP) is in the antibody-enzyme conjugate solution. The test sample is allowed to react simultaneously with the two antibodies, resulting in the CA125 molecules being sandwiched between the solid phase and enzymelinked antibodies. After incubation at 37°C for 90 minutes, the wells are washed with Wash Buffer to remove unbound-labeled antibodies. A solution of TMB Reagent is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of CA125 is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.Kit Components:Murine Monoclonal anti-CA125 coated microtiter plate with 96 wells.Enzyme Conjugate Reagent, 13mlStandard, 0unit/ml, 1x1mlStandard, 15unit/ml, 1x1mlStandard, 50unit/ml, 1x1mlStandard, 100unit/ml, 1x1mlStandard, 200unit/ml, 1x1mlStandard, 400unit/ml, 1x1ml Wash Buffer Concentrate (20X), 50mlTMB Reagent (One-Step), 11mlStop Solution (1N HCl), 11mlStorage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

產品特點: 針對Mucin 16, Human (MUC16, MUC-16, CA125, CA-125, Cancer Antigen 125, FLJ14303, Mucin 16 Cell Surface Associated, Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125) BioAssay ELISA Kit該產品的特點優(yōu)勢，歡迎查閱官網提供的產品說明書。

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其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。