Blocking Buffer for Fluorescent Western Blotting THIMEROSAL FREE 10-PACK (10 x 500 ml) - MB-070-010TF-Blocking Buffer for Fluorescent Western Blotting THIMEROSAL FREE 10-PACK (10 x 500 ml)

PRODUCT CENTER

生化試劑

產(chǎn)品中心

當(dāng)前位置：首頁(yè) > 產(chǎn)品中心 > 分子生化產(chǎn)品 > 生化試劑

中文名稱: Blocking Buffer for Fluorescent Western Blotting THIMEROSAL FREE 10-PACK (10 x 500 ml) - MB-070-010TF

英文名字: Blocking Buffer for Fluorescent Western Blotting THIMEROSAL FREE 10-PACK (10 x 500 ml) - MB-070-010TF

供應(yīng)商: Rockland

產(chǎn)品貨號(hào): MB-070-010TF

產(chǎn)品報(bào)價(jià): ￥詢價(jià)/10x500mL

產(chǎn)品說(shuō)明書(shū): 點(diǎn)擊查看

購(gòu)買方式: 90%產(chǎn)品中國(guó)有現(xiàn)貨庫(kù)存。銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868

產(chǎn)品新聞

背景資料: Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background.? IRDye?800, IRDye?700DX, Alexa Fluor? 680 and Cy5.5? antibody and reagent conjugates are specifically designed for protein detection methods that use longer-wavelength, near-infrared (IR) fluorophores to visualize proteins in western blotting and other applications.? IRDye?800 and IRDye?700DX fluoresce outside the range of most autofluorescence and therefore specific signal is sharply evident from any background giving the best possible signal-to-noise ratio.? Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film.? IRDye?800 conjugates are also suitable for immunofluorescence microscopy using commercially available excitation/emission filters in the 780nm/820nm range.? Dual simultaneous labeling in western blots or microscopy is achieved when IRDye?800 conjugates are used in conjunction with IRDye?700 or Cy5.5? conjugates. IRDye?800 and IRDye?700DX conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging. Once reacted with the membrane and dried, IRDye?800 and IRDye?700DX conjugated antibody-protein complexes are very stable, and membranes can be stored protected from light, re-washed and/or rescanned.? This blocking buffer is specifically formulated to achieve superior reproducible western blotting images using this system.

產(chǎn)品描述: Optimum performance is achieved using this product undiluted.? However, this blocking solution can often be diluted 1:1 in TBS without a significant loss of performance. This buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6.

產(chǎn)品形式: Liquid (sterile filtered)

保存建議: Store vial at 4° C prior to opening. DO NOT FREEZE.

其他: Rockland成立于1962年，是一家老牌抗體原產(chǎn)企業(yè)，其位于美國(guó)費(fèi)城郊區(qū)的農(nóng)場(chǎng)保有近20種實(shí)驗(yàn)動(dòng)物。Rockland公司為生命科學(xué)領(lǐng)域提供豐富的、高質(zhì)量的抗體，包括載脂蛋白、細(xì)胞周期/凋亡、細(xì)胞因子、細(xì)胞信號(hào)蛋白、酶類、細(xì)胞外基質(zhì)、轉(zhuǎn)錄因子(NFkB)和泛素（Ubiquitin）等識(shí)別抗體，應(yīng)用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產(chǎn)特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標(biāo)記二抗，應(yīng)用十分廣泛。