Our?Cholic?Acid ELISA Kit is designed for detection and?quantitation?of?cholic?acid in plasma, serum, urine, feces, or?lysates. This assay is a competitive ELISA where unknown samples are added to a plate pre-adsorbed with a?cholic?acid conjugate. An?anti-cholic?acid antibody is added, which is then detected by an?HRP?conjugated secondary antibody.?Cholic?acid levels in unknown samples are calculated based on a?cholic?acid standard curve.