NQO1, Human, BioAssay ELISA Kit (NAD(P)H Dehydrogenase [Quinone] 1, Azoreductase, DT-diaphorase, DTD, Menadione Reductase, NAD(P)H:quinone Oxidoreductase 1, Phylloquinone Reductase, Quinone Reductase -NQO1, Human, BioAssay ELISA Kit (NAD(P)H Dehydrogenase [Quinone] 1, Azoreductase, DT-diaphorase, DTD, Menadione Reductase, NAD(P)H:quinone Oxidoreductase 1, Phylloquinone Reductase, Quinone Reductase 1, QR1, DIA4, NMOR1)代理/參數(shù)/廠家-細(xì)胞分析試劑盒-艾美捷科技有限公司

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中文名稱: NQO1, Human, BioAssay ELISA Kit (NAD(P)H Dehydrogenase [Quinone] 1, Azoreductase, DT-diaphorase, DTD, Menadione Reductase, NAD(P)H:quinone Oxidoreductase 1, Phylloquinone Reductase, Quinone Reductase

英文名字: NQO1, Human, BioAssay ELISA Kit (NAD(P)H Dehydrogenase [Quinone] 1, Azoreductase, DT-diaphorase, DTD, Menadione Reductase, NAD(P)H:quinone Oxidoreductase 1, Phylloquinone Reductase, Quinone Reductase 1, QR1, DIA4, NMOR1)

供應(yīng)商: USBiological

產(chǎn)品貨號: N5379-52

產(chǎn)品報價: ￥1/100Tests

產(chǎn)品說明書: 點擊查看

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背景資料: USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨立的專業(yè)銷售團(tuán)隊、技術(shù)支持團(tuán)隊、市場營銷團(tuán)隊、進(jìn)出口報關(guān)團(tuán)隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價值的產(chǎn)品和服務(wù)，在意您的成功！

產(chǎn)品描述: NAD(P)H:quinone acceptor oxidoreductase 1 (NQO1), a multifunctional antioxidant enzyme and exceptionally versatile cytoprotector, both protects the cell from carcinogenic oxidative damage and stabilizes the tumor suppressor p53 protein. At high glycolysis levels NQO1 stabilizes and protects p53 from ubiquitin-independent degradation, a process that is NADH dependent, and also elevates NAD+/NADH levels. NAD+ and NADH play a crucial role in cellular energy metabolism, and a dysregulated NAD+/NADH ratio is implicated in metabolic syndrome. In humans, NQO1 is expressed at high levels in adipocytes and its expression levels are positively correlated with adiposity, glucose tolerance, and liver dysfunction. Thus NQO1 may provide the basis for a new therapy for the treatment of metabolic syndrome. This assay is a sandwich ELISA for quantitative determination of human NQO1 in cells. A monoclonal antibody specific for NQO1 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, NQO1 is recognized by the addition of a purified polyclonal antibody specific for NQO1 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of NQO1 in the samples. This ELISA is specific for the measurement of natural and recombinant human NQO1. It does not cross-react with human adiponectin, human RBP4, human Nampt, human vaspin, human progranulin, human resistin, human clusterin, human ANGPTL3, human CTRP5, human IL-33, human leptin, human GPX3, human NMNAT2, human sirtuin 1, human FTO, mouse Nampt, rat Nampt. The assay range is 0.313-20ng NQO1/ml and a detection limit of 100pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).Sample Type:Cell lysatesIntended Use:For the quantitative determination of NQO1 in cells.Sensitivity:100pg/ml Range:0.313-20ng NQO1/mlSpecificity:MammalianTest Principle:This assay is a sandwich ELISA for quantitative determination of human NQO1 in cells. A monoclonal antibody specific for NQO1 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, NQO1 is recognized by the addition of a purified polyclonal antibody specific for NQO1 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of NQO1 in the samples.Kit Components:Pre-coated Microtiter Plate: 12x8 well stripsWash Buffer (10X): 1x50mlDiluent (5X):1x50mlDetection Antibody: 1x12mlLysis Buffer (10X): 1x12mlDetector 100X (HRP conjugated anti-IgG): 1x150ulHuman NQO1 Standard (lyophilized): 40ngHuman NQO1 QC Sample (lyophilized): 1 vialSubstrate Solution (TMB): 1x12mlStop Solution: 1x12mlPlate Sealers: 3 eachStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

產(chǎn)品特點: 針對NQO1, Human, BioAssay ELISA Kit (NAD(P)H Dehydrogenase [Quinone] 1, Azoreductase, DT-diaphorase, DTD, Menadione Reductase, NAD(P)H:quinone Oxidoreductase 1, Phylloquinone Reductase, Quinone Reductase 1, QR1, DIA4, NMOR1)該產(chǎn)品的特點優(yōu)勢，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。

保存建議: 建議收到NQO1, Human, BioAssay ELISA Kit (NAD(P)H Dehydrogenase [Quinone] 1, Azoreductase, DT-diaphorase, DTD, Menadione Reductase, NAD(P)H:quinone Oxidoreductase 1, Phylloquinone Reductase, Quinone Reductase 1, QR1, DIA4, NMOR1)產(chǎn)品后將其置于4°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。