用于開發(fā)夾心ELISA試劑盒，以測定細胞培養(yǎng)上清液、血清和血漿（肝素、EDTA、檸檬酸鹽）中的人GM-CSF。-For the development of sandwich ELISA kit to measure human GM-CSF in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).代理/參數(shù)/廠家-ELISA試劑盒-艾美捷科技有限公司

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英文名字: For the development of sandwich ELISA kit to measure human GM-CSF in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

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背景資料: Granulocyte-macrophage colony-stimulating factor(GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.

產(chǎn)品描述: 粒細胞-巨噬細胞集落刺激因子（GM-CSF）也用CSF 2表示。人GM-CSF是前體細胞體外增殖和分化為成熟粒細胞和巨噬細胞所必需的糖蛋白。人cDNA克隆含有編碼144個氨基酸的蛋白質(zhì)的單一開放閱讀框，預測分子量為16，293道爾頓，并顯示與小鼠GM-CSF的69%核苷酸同源性和54%氨基酸同源性。人GM-CSF基因似乎以單拷貝基因形式存在。人GM-CSF是一種22，000道爾頓的糖蛋白，其刺激骨髓祖細胞的生長并直接作用于成熟的中性粒細胞。GM-CSF基因通過體細胞雜交分析和原位雜交定位于人染色體區(qū)域5 q21 - 5 q32，其涉及5 q-綜合征和急性髓性白血病的間質(zhì)缺失。T淋巴細胞衍生的淋巴因子GM-CSF的互補DNA已被克隆，重組GM-CSF蛋白已在酵母中表達并純化至同質(zhì)。這種純化的人重組GM-CSF在體外刺激外周血單核細胞對惡性黑素瘤細胞系A(chǔ)375具有細胞毒性。

其他: Nordic BioSite(北歐生物) 總部位于瑞典，成立于1997年，旨在分銷研究和診斷領(lǐng)域的高質(zhì)量和創(chuàng)新產(chǎn)品。 Nordic BioSite 被公認為整個北歐生物醫(yī)學研究和診斷產(chǎn)品領(lǐng)域的領(lǐng)導者。他們擁有超過570萬種的產(chǎn)品組合，代理了遍布歐洲和美國的頂級生物試劑制造商，是歐洲知名的一站式生物試劑代理商。除了作為分銷商之外，Nordic BioSite 還擁有不斷增長的自產(chǎn)產(chǎn)品線——BioSite mAb、BioSite ELISA、BioSite Flow、Optibodies和 BioSite Protein, 目前艾美捷代理的是Nordic BioSite自產(chǎn)產(chǎn)品線(共26W+產(chǎn)品)。Nordic BioSite的產(chǎn)品類型主要是抗體，蛋白和試劑盒，產(chǎn)品適用范圍涵蓋15個不同的物種(人，牛，犬，鯰魚，雞，海豚，馬，貓，豚鼠，小鼠，綿羊，兔子，大鼠，豬和火雞)，可為您提供一站式的生命科學研究解決方案~