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細(xì)胞分析試劑盒
中文名稱(chēng)

血漿/血清循環(huán)核酸純化大提試劑盒（提取試劑盒）

英文名字
Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit (Slurry Format)
供應(yīng)商
Norgen Biotek
產(chǎn)品貨號(hào)
NGB-53400
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產(chǎn)品描述
Norgen's Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit provides a fast, reliable and simple procedure for isolating circulating nucleic acids (mRNA, miRNA, exosomal RNA, viral RNA, DNA and viral DNA) from various amounts of plasma/serum ranging from 2 mL to 5 mL.This kit is able to isolate all sizes of circulating RNA, including microRNA, as well as all sizes of exosomal RNA and circulating DNA. Norgen’s Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating NA from large sample volumes. Free-Circulating NA can be isolated from either fresh or frozen samples using this kit, and the kit allows for the concentration of NA that is present in low concentrations (1-100ng/mL in human plasma). Typical yields of free-circulating NA will vary depending on the input sample, as the amount of NA present in plasma and serum will depend upon the health status of the individual and the level of nucleases present in the blood. This kit is suitable for the purification of Nucleic Acid from serum or plasma prepared from blood collected on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.Free-circulating nucleic acids in plasma and serum have the potential to provide biomarkers for certain cancers and disease states, and include tumor-specific extracellular nucleic acid fragments and fetal DNA in maternal blood. Free-circulating DNA is usually present as short fragments of less than 1000 bp, whereas free-circulating RNA and exosomal RNA in plasma and serum are usually present as short fragments of less than 1000 nt. In addition, plasma or serum also contains free-circulating miRNA that are as small as 21 nt. Furthermore, circulating viral DNA or viral RNA may be present in the plasma or the serum of viral-infected patients. Norgen’s Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit provides an efficient method for the purification of all sizes of these fragmented free-circulating nucleic acids from human plasma or serum.
產(chǎn)品特點(diǎn)
Isolate all sizes of circulating and exosomal RNA, including microRNA; Isolate all sizes of circulating DNA from plasma and serum samples; Versatile plasma and serum input volumes (2 mL to 5 mL); Concentrate circulating nucleic acids into small elution volumes; Isolate inhibitor-free circulating nucleic acids; Bind and elute all RNA irrespective of size or GC content, without bias.
保存建議
所有緩沖液應(yīng)密封保存，并在室溫（15-25°C）下保存長(zhǎng)達(dá)1年。具體保存情況收到產(chǎn)品后請(qǐng)按說(shuō)明書(shū)存放。
其他
Norgen Biotek成立于1998年，公司總部位于加拿大，是一家著名全球化生物技術(shù)公司，基于專(zhuān)利的碳化硅膜純化技術(shù)和無(wú)苯酚專(zhuān)利配方，生產(chǎn)高品質(zhì)的核酸和蛋白質(zhì)純化及富集產(chǎn)品用于研究和診斷。Norgen Biotek 在2003年獲得加拿大國(guó)家研究委員會(huì)頒發(fā)的科技創(chuàng)新獎(jiǎng)，是一家致力于樣品制備并獲得ISO 9001（產(chǎn)品質(zhì)量）、ISO 13485（產(chǎn)品可用于商業(yè)化的醫(yī)療設(shè)備，包括用于體外診斷領(lǐng)域）和ISO 15189（可用于臨床檢測(cè)機(jī)分子診斷領(lǐng)域的研發(fā)能力）認(rèn)證的生物科技公司。 目前該公司的產(chǎn)品多達(dá)700余種，提供一流的樣品制備試劑盒，應(yīng)用于基因組和蛋白組等不同的領(lǐng)域。其產(chǎn)品主要覆蓋包括各種標(biāo)本類(lèi)型的DNA，RNA和蛋白質(zhì)的純化富集，從活檢樣本（包括尿液，血漿，血清和血液）中純化RNA和DNA，全系列的PCR試劑、緩沖液、分子量ladder和塑料過(guò)濾裝置。
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