EpiQuik通用三甲基化組蛋白H4-K20定量分析試劑盒(比色法)專(zhuān)門(mén)為檢測(cè)總體組蛋白H4-K20的三甲基化而設(shè)計(jì).檢測(cè)實(shí)驗(yàn)中,而第20位賴(lài)氨酸被三甲基化的組蛋白H4結(jié)合到表面吸附了抗三甲基化組蛋白H4-K20抗體的微孔上.被捕獲的三甲基組蛋白H4-K20被結(jié)合了一種顯色劑的特異性抗體識(shí)別.三甲基化組蛋白H4-K20的量與吸光度成正比,其總量可以通過(guò)對(duì)比對(duì)照組來(lái)測(cè)定.該通用三甲基化組蛋白H4-K20定量試劑盒(比色法)內(nèi)主要產(chǎn)品組份包括:C1 (10X wash buffer)
C2 (antibody buffer)
C3 (detecting antibody, 1 mg/ml)
C4 (color developer)
C5 (stop solution)
Standard control (100 ug/ml)
Signal report solution
Signal enhancer
8 well sample strips (with frame)
8 well standard control strips
用戶(hù)指南手冊(cè)
已發(fā)表的部分文獻(xiàn): 標(biāo)題:Histone Methylation Marks on Circulating Nucleosomes as Novel Blood-Based Biomarker in Colorectal Cancer作者:Gezer U, Ebru E. Y??r??ker 1, Metin Keskin 2, Cemil Burak Kulle 2, Yoganiranjana Dharuman 3 and Stefan Holdenrieder 3;雜志名稱(chēng):Int J Mol Sci 標(biāo)題:Plasma Histone H4 and H4K20 Trimethylation Levels Differ Between Colon Cancer and Precancerous Polyps.作者:?zgür E, Keskin M, Y?rüker EE, Holdenrieder S, Gezer U. ;雜志名稱(chēng):In Vivo 標(biāo)題:Epigenetic regulations in alternative telomere lengthening: Understanding the mechanistic insight in arsenic-induced skin cancer patients.作者:Bhattacharjee P, Das A, Giri AK, Bhattacharjee P;雜志名稱(chēng):Sci Total Environ 標(biāo)題:Perinatal inflammation alters histone 3 and histone 4 methylation patterns: Effects of MiR-29b supplementation.作者:Sugar SS, Heyob KM, Cheng X, Lee RJ, Rogers LK;雜志名稱(chēng):Redox Biol 標(biāo)題:Advances in liquid biopsy in neuroblastoma.作者:Zhuo Z, Lin L, Miao L, Li M, He J;雜志名稱(chēng):Fundam Res Epigentek 公司是全球領(lǐng)先的表觀遺傳學(xué)相關(guān)研究的技術(shù)創(chuàng)新者和產(chǎn)品開(kāi)發(fā)供應(yīng)商。開(kāi)發(fā)了超過(guò)700多個(gè)專(zhuān)利產(chǎn)品,為表觀遺傳學(xué)方面的研究和新藥研發(fā)提供全面系統(tǒng)的解決方案,涵蓋樣品制備、DNA/RNA甲基化(5-mc, 5-hmc, 5-fc, m6A)/DNMT分析、ChIP、組蛋白甲基化/乙酰化/去乙酰化/磷酸化/SUMO-等領(lǐng)域,提供小分子抑制劑、蛋白質(zhì)、抗體、以及分析試劑盒產(chǎn)品。