Visfatin, an adipocytokine that is highly enriched in the visceral fat of both humans and mice and whose expression level in plasma increases during the development of obesity. Visfatin corresponds to pre-B cell colony-enhancing factor (PBEF), a 52kD cytokine expressed in lymphocytes. PBEF is an inflammatory cytokine that plays a requisite role in the delayed neutrophil apoptosis of sepsis. Visfatin exerted insulin-mimetic effects in cultured cells and lowered plasma glucose levels in mice. It was found that visfatin binds to and activates the insulin receptor. Plasma level of visfatin in patients with type 2 diabetes mellitus was elevated, suggesting that measurement of plasma visfatin provides a relevant tool for understanding metabolic diseases.Components:1) Antibody coated 96-well plate (12 x 8 well strips, with absorbed monoclonal antibody to human visfatin)2) 5X Wash concentrate, 100ml 3) 5X Diluent, 50ml (for reagent dilution)4) 1X Secondary antibody, 12ml (Rabbit Polyclonal antibody against human visfatin) 5)100X Detector, 150ul (HRP conjugated anti-rabbit IgG)6) Standard, recombinant human visfatin (16ng), lyophilized 7) QC sample= 2 positive control of recombinant human visfatin (3ng/ml, 0.2ng/ml) 8) Substrate, 12ml (chromogenic reagent)9) Stop solution, 12ml (1M H3PO4)10) Plate sealer (3 sealers)Storage and Stability:Store at 4°C. Store powder at 4°C liquid at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.