- 中文名稱(chēng)
Peroxidase Assay Kit, BioAssay, Colorimetric/Fluorometric
- 英文名字
- Peroxidase Assay Kit, BioAssay, Colorimetric/Fluorometric
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號(hào)
- P3349-91
- 產(chǎn)品報(bào)價(jià)
- ￥1/96Tests
- 產(chǎn)品說(shuō)明書(shū)
- 點(diǎn)擊查看
- 購(gòu)買(mǎi)方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類(lèi)最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國(guó)區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專(zhuān)業(yè)銷(xiāo)售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營(yíng)銷(xiāo)團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢(xún)響應(yīng)，專(zhuān)業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！
- 產(chǎn)品描述
- Peroxidases (EC number 1.11.1.x) catalyze the following oxidationreduction reactions: ROOR'+electron donor (2 e-)+2H+ → ROH+R'OH For many peroxidases the optimal substrate is hydrogen peroxide (H2O2), but others are more active with organic hydroperoxides such as lipid peroxides. In the cell, peroxidases destroy toxic hydroxide radicals that are formed as byproducts during aerobic respiration. The peroxidases represent a large family of enzymes that are found in animals (e.g. myeloperoxidase-like enzymes), plant, fungi and bacteria (cytochrome-c peroxidase like enzymes such a horseradish peroxidase).Simple, direct and automation-ready procedures for determining peroxidase activity find wide Applications: Peroxidase assay uses H2O2 and an electron donor dye that forms a pink color during the peroxidase reaction. The optical density (570nm) or fluorescence intensity (lexc=530nm, lem=590nm) is a direct measure of the enzyme activity.Key Features:Use as little as 10ul samples. Linear detection range: Colorimetric Assay:s 4 to 1000 IU/L, Fluorometric Assay:s 0.8 to 25 IU/L peroxidase.Kit Contents:Assay Buffer (pH 7.0): 20ml Dye Reagent: 60ulStabilized H2O2: 100ul 3% S top Reagent: 12mlCalibrator: 5ml (equivalent to 1000 IU/L)Storage conditions. The kit is shipped on blue ice. Store Assay Buffer and Dye Reagent at -20°C, all other reagents at 4°C. Shelf life: 3 months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:Sample Preparation. Samples can be prepared according to established methods [1-3]. It is prudent to test multiple sample dilutions to ensure activity is in the linear range.Reagent Preparation: Bring all reagents to room temperature prior to assay. Dilute 3% H2O2 in Assay Buffer to 0.6% and use within one hour.96-Well Colorimetric Assay: Procedure:. Use clear flat-bottom plates for Colorimetric Assay:s1. Transfer 200ul H2O and 200ul Calibrator into two wells of a clear flat-bottom 96-well plate. Transfer 10ul H2O (sample blank), 10ul sample to separate wells. Prepare fresh Working Reagent for each reaction well by mixing 95ul Assay Buffer, 0.5ul Dye Reagent and 0.5ul freshly diluted 0.6% H2O2. Add 90ul Working Reagent to each sample well. Tap plate to mix and incubate for 10 min at room temperature.2. Add 100ul Stop Reagent to the sample blank and sample wells. Tap plate to mix and read OD570nm.Note: if Sample OD values are higher than that of the Calibrator, dilute sample in Assay Buffer, repeat assay and multiply results by the dilution factor. Peroxidase activity is calculated from the OD values of the sample, sample blank, calibrator and H2O wells.Peroxidase Activity=x 1000 (IU/L)ODSAMPLE–ODBLANKODCALIBRATOR–ODH2OUnit definition: one unit of enzyme will catalyze the oxidization by H2O2 of 1uMole dye reagent per min under the assay conditions.96-Well Fluorometric Assay: Procedure:. Use black flat-bottom plates. If desirable, a peroxidase standard can be run together with the samples.1. Transfer 10ul H2O, 10ul sample to separate wells. Prepare fresh Working Reagent for each sample well by mixing 95ul Assay Buffer, 0.5ul Dye Reagent and 0.5ul freshly diluted 0.6% H2O2. Add 90ul Working Reagent to each well. Tap plate to mix and incubate for 10 min at room temperature.2. Add 100ul Stop Reagent to the sample blank and sample wells. Tap plate to mix and read fluorescence at 590nm (lexc= 530nm).384-Well Fluorometric Assay: Procedure:. Use 5ul H2O, 5ul sample, 35ul Working Reagent prepared from 38ul Assay Buffer, 0.2ul Dye Reagent, 0.2ul 0.6% H2O2. Use 40ul Stop Reagent.General Considerations:This assay is based on a kinetic reaction, the use of a multi-channel pipettor for adding the Working Reagent and Stop Reagent is recommended.Materials Required, But Not Provided:Pipeting devices, centrifuge tubes, clear flat bottom 96/384-well plates for Colorimetric Assay:s, black 96/384-well plate for Fluorometric Assay:s and plate reader.
- 產(chǎn)品特點(diǎn)
- 針對(duì)Peroxidase Assay Kit, BioAssay, Colorimetric/Fluorometric該產(chǎn)品的特點(diǎn)優(yōu)勢(shì)，歡迎查閱官網(wǎng)提供的產(chǎn)品說(shuō)明書(shū)。
- 保存建議
- 建議收到Peroxidase Assay Kit, BioAssay, Colorimetric/Fluorometric產(chǎn)品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國(guó)著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類(lèi)最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過(guò)50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁(yè)面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說(shuō)明書(shū)為準(zhǔn)。下單前，請(qǐng)瀏覽說(shuō)明書(shū)確認(rèn)。
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