- 中文名稱
Progesterone BioAssay ELISA Kit
- 英文名字
- Progesterone BioAssay ELISA Kit
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號(hào)
- P9006-08
- 產(chǎn)品報(bào)價(jià)
- ￥1/1Kit
- 產(chǎn)品說(shuō)明書(shū)
- 點(diǎn)擊查看
- 購(gòu)買(mǎi)方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類(lèi)最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國(guó)區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專業(yè)銷(xiāo)售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營(yíng)銷(xiāo)團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！
- 產(chǎn)品描述
- Progesterone is a C-21 steroid secreted by the corpus luteum and the placenta. It is an important intermediate in steroid biosynthesis in all tissues that secrete steroid hormones. Progesterone is responsible for the progestational changes in the endometrium and cyclic changes in the cervix and vagina. The plasma progesterone level is useful in investigating the luteal function and monitoring ovulation.Principle of the Assay:This is an ELISA for the quantitative analysis of Progesterone levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the Progesterone in the sample for a limited number of binding sites on the antibody coated plate.The sample or standard solution is first added to the microplate. Next, the diluted enzyme conjugate is added and the mixture is shaken and incubated at room temperature for one hour. During the incubation, competition for binding sites is taking place. The plate is then washed removing all the unbound material. The bound enzyme conjugate is detected by the addition of substrate, which generates an optimal color after 30 minutes.Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650nm. The extent of color development is inversely proportional to the amount of Progesterone in the sample or standard. For example, the absence of Progesterone in the sample will result in a bright blue color, whereas the presence of Progesterone will result in decreased or no color development.Sample Preparation:Usually, urine and tissue culture supernatant can be assayed directly by diluting them with the diluted extraction buffer. Plasma and most other mediums will need to be extracted.Range: 0.4-40.0ng/mlKit Components:P9006-08A: ELISA Buffer 1x30ml. To be used to dilute enzyme conjugate and Progesterone standards.P9006-08B: Wash Buffer (10X) 1x20ml. To be diluted 10x with deionized water. This is used to wash all unbound enzyme conjugate, samples and standards from the plate after the one hour incubation.P9006-08C: Substrate 1x20ml. Stabilized 3,3', 5,5' Tetramethylbenzidine (TMB) plus hydrogen peroxide (H2O2) in a single bottle. It is used to develop the color in the wells after they have been washed.P9006-08D: Extraction Buffer (5x) 1x30ml. To be diluted 5x with deionized water. This is used for diluting extracted and non-extracted samples.P9006-08E: Progesterone (HRP) 1x150ul. Progesterone HRP concentrate.P9006-08F: Progesterone Standard 1x100ul. Progesterone standard at the concentration of 1ug/ml. Green capped vial.P9006-08G: Microplate 1x96 well microplate with anti-Progesterone rabbit antibody precoated on each well. The plate is ready for use as is. Do not wash.Storage and Stability:Store at 4°C. Do Not Freeze. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產(chǎn)品特點(diǎn)
- 針對(duì)Progesterone BioAssay ELISA Kit該產(chǎn)品的特點(diǎn)優(yōu)勢(shì)，歡迎查閱官網(wǎng)提供的產(chǎn)品說(shuō)明書(shū)。
- 保存建議
- 建議收到Progesterone BioAssay ELISA Kit產(chǎn)品后將其置于4°C保存。
- 其他
- Usbiological公司是美國(guó)著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類(lèi)最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過(guò)50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁(yè)面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說(shuō)明書(shū)為準(zhǔn)。下單前，請(qǐng)瀏覽說(shuō)明書(shū)確認(rèn)。
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