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細胞分析試劑盒
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中文名稱

Prostaglandin E2 (PGE2) BioAssay ELISA Kit

英文名字
Prostaglandin E2 (PGE2) BioAssay ELISA Kit
供應(yīng)商
USBiological
產(chǎn)品貨號
P9053-29
產(chǎn)品報價
¥1/1Kit
產(chǎn)品說明書
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購買方式
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產(chǎn)品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理,是行業(yè)中少有的致力于服務(wù)客戶,幫助客戶,且擁有獨立的專業(yè)銷售團隊、技術(shù)支持團隊、市場營銷團隊、進出口報關(guān)團隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應(yīng),專業(yè)的產(chǎn)品和解決方案支持,穩(wěn)健快捷的交貨周期,優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價值的產(chǎn)品和服務(wù),在意您的成功!
產(chǎn)品描述
Prostaglandin E2 (PGE2) is derived from PGH2 which in turn is synthesized from arachidonic acid through the cyclooxygenase pathway. Many cell types, such as epithelial cells, fibroblasts, and macrophages produce PGE2. PGE2 possesses vasoactivity, modulates immune funtions, regulates sleep-awake cycles and exhibits many other activities.Principle Of The Assay:This is an polyclonal ELISA (Enzyme-Linked Immunosorbent Assay) for the quantitative analysis of Prostaglandin E2 levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the PGE2 in the sample for a limited number of binding sites on the antibody coated plate. The sample or standard solution is first added to the microplate. Next, the diluted enzyme conjugate is added and the mixture is shaken and incubated at room temperature for one hour. During the incubation, competition for binding sites is taking place. The plate is then washed removing all the unbound material. The bound enzyme conjugate is detected by the addition of substrate, which generates an optimal color after 30 minutes. Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650nm. The extent of color development is inversely proportional to the amount of PGE2 in the sample or standard. For example, the absence of PGE2 in the sample will result in a bright blue color, whereas the presence of PGE2 will result in decreased or no color development.Kit Components:P9053-29A: EIA Buffer, 1x30mlP9053-29B: Wash Buffer (10X), 1x20mlP9053-29C: TMB Substrate, 1x20mlStabilized 3,3', 5,5' Tetramethylbenzidine (TMB) plus hydrogen peroxide (H2O2) in a single bottle.P9053-29D: Extraction Buffer (5X), 1x30mlP9053-29E: Prostaglandin E2 (HRP), 1x150ulP9053-29F: Prostaglandin E2 Standard (1ug/ml), 1x100ulP9053-29G: Microtiter Plate, 1x96wells Precoated anti-PGE2 rabbit antibody. Ready to use.Storage and Stability:Store all components at 4°C Stable for 6 months after receipt.
產(chǎn)品特點
針對Prostaglandin E2 (PGE2) BioAssay ELISA Kit該產(chǎn)品的特點優(yōu)勢,歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
保存建議
建議收到Prostaglandin E2 (PGE2) BioAssay ELISA Kit產(chǎn)品后將其置于4°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品,為科研用戶提供了諸多超值選擇。
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