Prostaglandin F2 alpha, 11-Beta (PGF2alpha) BioAssay ELISA Kit-Prostaglandin F2 alpha, 11-Beta (PGF2alpha) BioAssay ELISA Kit代理/參數(shù)/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: Prostaglandin F2 alpha, 11-Beta (PGF2alpha) BioAssay ELISA Kit

英文名字: Prostaglandin F2 alpha, 11-Beta (PGF2alpha) BioAssay ELISA Kit

供應商: USBiological

產品貨號: P9053-34D

產品報價: ￥1/1Kit

產品說明書: 點擊查看

購買方式: 銀行轉賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868

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產品描述: 11b-Prostaglandin F2a (11b-PGF2a) is derived from PGD2 by the action of 11-ketoreductase. It is a potent broncho-constrictor agent. It also causes hypertension in animals. Plasma levels of 11b-PGF2a are highly elevated in patients with systemic mastocytosis with an overproduction of PGD2.Principle of the Assay:This is an ELISA (Enzyme-Linked Immunosorbent Assay) for the quantitative analysis of 11b-Prostaglandin F2a levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the 11b-PGF2a in the sample for a limited number of binding sites on the antibody coated plate. The sample or standard solution is first added to the microplate. Next, the diluted enzyme conjugate is added and the mixture is shaken and incubated at room temperature for one hour. During the incubation, competition for binding sites is taking place. The plate is then washed removing all the unbound material. The bound enzyme conjugate is detected by the addition of substrate which generates an optimal color after 30 minutes. Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650nm. The extent of color development is inversely proportional to the amount of 11b-PGF2a in the sample or standard. For example, the absence of 11b-PGF2a in the sample will result in a bright blue color, whereas the presence of 11b-PGF2a will result in decreased or no color development.Kit Components:1. EIA Buffer, 1x30ml. To be used to dilute enzyme conjugate and 11b-PGF2a standards.2. Wash Buffer (10X), 120ml. To be diluted 10x with deionized water. This is used to wash all unbound enzyme conjugate, samples and standards from the plate after the one hour incubation.3. Substrate, 1x20ml. Stabilized 3,3', 5,5' Tetramethylbenzidine (TMB) plus Hydrogen Peroxide (H2O2) in a single bottle. It is used to develop the color in the wells after they have been washed.4. Extraction Buffer (5X), 1x30ml. To be diluted 5x with deionized water. This is used for diluting extracted and non-extracted samples.5. 11-Prostaglandin F Enzyme Conjugate, 1x150ul. 11b-PGF2a HRP concentrate. Blue capped vial.6. 11-Prostaglandin F Standard, 1x100ul. 11b-PGF2a standard at the concentration of 1ug/ml. Green capped vial.7. 11-Prostaglandin F2 Antibody Coated Plate, 1xA 96-well microplate with anti-11b-PGF2a rabbit antibody precoated on each well. The plate is ready for use as is. Do not wash.Storage and Stability:Store components at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.?

產品特點: 針對Prostaglandin F2 alpha, 11-Beta (PGF2alpha) BioAssay ELISA Kit該產品的特點優(yōu)勢，歡迎查閱官網(wǎng)提供的產品說明書。

保存建議: 建議收到Prostaglandin F2 alpha, 11-Beta (PGF2alpha) BioAssay ELISA Kit產品后將其置于-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。