- 中文名稱
Protein Kinase C Assay Kit, BioAssay (PKC) discontinued
- 英文名字
- Protein Kinase C Assay Kit, BioAssay (PKC) discontinued
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號(hào)
- P9105-98
- 產(chǎn)品報(bào)價(jià)
- ￥1/1Kit
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營銷團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！
- 產(chǎn)品描述
- The assay kit is designed to measure the phosphotransferase activity of protein kinase C (PKC) in immunoprecipitates and column fractions. It is highly specific for alpha, beta and gamma. Crude cell lysates may also be used but detergents/biochemicals contained in the cell lysis buffer may inhibit PKC kinase activity. Furthermore, although two inhibitors are included with the kit, editors may suggest other unknown kinases found in crude lysates are responsible for substrate peptide phosphorylation. The assay kit is based on phosphorylation of a specific substrate peptide (QKRPSQRSKYL) using the transfer of the -phosphate of adenosine-5'-[32P] triphosphate ([ -32P] ATP) by PKC kinase. The phosphorylated substrate is then separated from the residual [ -32P] ATP using P81 phosphocellulose paper and quantitated by using a scintillation counter. The assay is linear for incubation times of up to 30 minutes and incorporation of up to 20% of total ATP. Further incubation or incorporation may not be linear and may therefore not be a true indication of PKC activity in the sample extract. The enzyme assay is rapid, convenient and fairly specific for PKC. Capacity:~200 PKC kinase assays. Kit Components: 1. Assay Dilution Buffer II (ASBII) containing 20mM MOPS, pH 7.2, 25mM β-glycerol phosphate, 1mM sodium orthovanadate, 1mM dithiothreitol, 1mM CaCl , 2x1ml2. PKC Substrate Cocktail, 500uM PKC substrate peptide [QKRPSQRSKYL] in ADBII, 2x1ml3. PKA/CaMK Inhibitor Cocktail containing 2uM PKA inhibitor peptide, and 20uM Compound R24571 in ADBII, 2x1ml4. PKC Lipid Activator containing 0.5mg/ml phosphatidyl serine and 0.05mg/ml diacylglycerol in ADBII, 2x1ml5. Mg/ATP Cocktail containing 75mM magnesium chloride and 500um ATP 20mM MOPS, pH 7.2, 25mM β-glycerophosphate, 5mM EGT A, 1mM Na3VO4, 1mM dithiothreitol, 2x1ml. (Before starting the assay, 90ul of the Mg2+/ATP cocktail should be added to 100uCi (10ul) of the [γ-32P]ATP (～3000Ci/mmol).)6. p81 squares, 1x200 eachStorage and Stability:Store components at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.?Other components required but not included as part of kit are: Sample preparation containing Protein Kinase C: 10-200ug protein/immunoprecipitate diluted into assay dilution buffer or 25-100ng of purified Protein Kinase C vortex mixer plexiglass shielding 30°C incubating water bath timer variable volume (5-200ul) pipet + tips 0.75% phosphoric acid scintillation vials scintillation fluid scintillation counter [gamma-32P]ATP-3000 Ci/mmol,
- 產(chǎn)品特點(diǎn)
- 針對(duì)Protein Kinase C Assay Kit, BioAssay (PKC) discontinued該產(chǎn)品的特點(diǎn)優(yōu)勢(shì)，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
- 保存建議
- 建議收到Protein Kinase C Assay Kit, BioAssay (PKC) discontinued產(chǎn)品后將其置于-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說明書為準(zhǔn)。下單前，請(qǐng)瀏覽說明書確認(rèn)。
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