Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes. All VEGF-receptors have seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. VEGFR-2 has a lower affinity for VEGF than the Flt-1 receptor, but a higher signaling activity. Mitogenic activity in endothelial cells is mainly mediated by VEGFR-2 leading to their proliferation.
Differential splicing of the flt-1 gene leads to the formation of a secreted, soluble variant of VEGFR-1 (sVEGFR-1). No naturally occuring, secreted forms of VEGFR-2 have so far been reported. The binding of VEGF165 to VEGFR-2 is dependent on heparin.
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來源宿主
昆蟲細(xì)胞中重組表達(dá)
溶解建議
提供的人血管內(nèi)皮生長因子受體-2(VEGFR2)產(chǎn)品為過濾滅菌的白色凍干粉末;該凍干粉末是從含1mg/ml人血管內(nèi)皮生長因子受體-2(VEGFR2)的25mM MES pH 5.5,100mM NaCl溶液中過濾滅菌并凍干產(chǎn)生的.我們建議加無菌水以配制工作儲存液到不低于100ug/ml,后繼實(shí)驗(yàn)過程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋.