Osteoprotegerin (OPG) is a member of the TNFR superfamily that can act as a decoy receptor for RANKL. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. OPG is expressed in a wide variety of tissues including adult heart, lung, kidney, liver, spleen, prostate, lymph node and bone marrow. OPG is secreted both as a monomeric and a dimeric protein. Its primary structure consists of seven distinct domains, four of which corresponds to the extracellular cysteine-rich domains of TNFR proteins and constitutes the soluble OPG.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20?C. Further dilutions should be made in appropriate buffered solutions.
保存建議
This lyophilized preparation is stable at 2-8?C, but should be kept at -20?C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8?C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20?C to -70?C. Avoid repeated freeze/thaw cycles.