POU5F1 is a homeodomain transcription factor of the POU family, expressed in embryonic stem (ES) cells and embryonic carcinoma (EC) cells.POU5F1 is significantly involved in the signaling pathway for maintaining self-renewal and pluripotency of ES cells. POU5F1 has 2 distinct DNA binding domains which independently bind half-sites of the canonical octamer motif. This flexibility allows POU5F1 to bind with distinct DNA motifs by forming heterodimers with other transcription factors or by forming homodimers in several conformations. Human POU5F1 contains a 75aa POU specific (POUS) domain and a 60aa POU-Homeo-(POUH) domain connected by a linker region. The Human POU5F1 specifically interacts with Octamer motif ATGCAAAT. In addition, 2 proline-rich domains in the N-terminal and C-terminal regions are vital for POU5F1 transactivation. POU5F1 regulates a number of target genes and has been shown to work jointly with other transcription factors including Sox2 as well as Nanog to sustain stem cell potency and self-renewal.
序列
請咨詢我們客服或者請查看品牌官方網(wǎng)站
來源宿主
大腸桿菌中重組表達(dá)
溶解建議
提供的重組人POU class 5 同源框1產(chǎn)品形式為經(jīng)無菌過濾處理的無色溶液.
保存建議
重組人POU class 5 同源框1產(chǎn)品在4℃保存請2 - 4周內(nèi)將使用;-20℃可長期保存.產(chǎn)品若長期保存,建議添加載體蛋白(0.1% HSA或BSA).避免反復(fù)凍融.