Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is wilyde used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10**14). The complexes are also extremely stable over a wide range of temperature and pH.
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來源宿主
細菌中純化的鏈霉親生物素蛋白
溶解建議
Sterile Filtered lyophilized powder. Lyophilized in 10mM potassium phosphate buffer pH 6.5. Gives a clear solution at 5mg/ml in 0.1M NaCl.
保存建議
Streptavidin although stable at 4℃ for 3 weeks, should be stored desiccated below -18℃.
For longer storage in dissolved form add 1mM EDTA and/or 0.02 % NaN3 or pass the solution through a sterile filter.
Please prevent freeze-thaw cycles.