中文名稱

【D4GDI】重組人D4-GDI蛋白

英文名字

Recombinant Human D4-GDI

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ProSpec

產(chǎn)品貨號

PRO-296

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產(chǎn)品新聞

背景資料

D4-GDI belongs to the family of Rho-GDIs or GDP-dissociation inhibitors for the Rho/Rac family of small G proteins； consisting of RhoGDI-1， D4 GDI/RhoGDI-2 and RhoGDI-3. It inhibits the release of GDP from the Rho proteins， essential for GTP-loading and activation of GTPase activity. D4-GDI is located in the cytoplasm and is involved in the regulation of the membrane association and dissociation cycle of Rho/Rac proteins. It is known to be a substrate for caspase 3 during Fas-mediated apoptosis.
D4-GDI is expressed only in hematopoietic tissues， mainly in B and T cells， unlike RhoGDI which is expressed ubiquitously. D4-GDI plays a role in the regulation of lymphocyte activation， survival， and responsiveness. Expression of D4-GDI is also observed in cells of nonhematopoietic neoplasms， including ovarian and bladder cancer cells， suggestive of D4-GDI’s role in progression of human tumors. D4-GDI is expressed in human breast cancer cells. In different human breast cancer cell lines that were studied， cell lines with high invasive activities， for example MDA-MB-231， expressed higher D4-GDI than did weakly invasive and noninvasive cell lines. Intentional disruption of D4-GDI by steadily expressing small interfering RNA （siRNA） against D4-GDI transcripts successfully blocks the motility and invasive potential of MDA-MB-231 cells in vitro. Cells deficient of D4-GDI revert to a normal breast epithelial phenotype characterized by the formation of cysts with a central lumen when grown on Matrigel. D4-GDI reduction is linked to a down-regulation of the cell surface protein ?1-integrin and a loss of cell-matrix adhesion， suggestive of D4-GDI induction of cell invasion by controlling ?1-integrin expression. The expression of ?1-integrin and invasive activities are restored once there’s restitution of D4-GDI expression to the levels found in the parental MDA-MB-231 cells. Therefore it is suggested that D4-GDI may play a significant role in the regulation of breast cancer cell invasiveness.

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來源宿主

大腸桿菌中重組表達(dá)

溶解建議

Sterile Filtered clear solution. D4-GDI protein in 50mM Tris-acetate， pH7.5， 20% glycerol and 1mM EDTA.

保存建議

Store vial at -20℃ to -80℃. When stored at the recommended temperature， this protein is stable for 12 months. Please prevent freeze-thaw cycles.

其他

ProSpec 是一家全球著名細(xì)胞因子蛋白生產(chǎn)和研發(fā)的公司，具有20多年的生產(chǎn)經(jīng)驗(yàn)，專注于蛋白（重組及合成）生產(chǎn)研發(fā)。我們的核心產(chǎn)品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長因子、瘦素、干細(xì)胞因子、催乳素、表皮生長因子、胰島素樣生長因子、神經(jīng)生長因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。 領(lǐng)先的生產(chǎn)工藝和規(guī)模使其可以提供毫克到克級蛋白，價(jià)格優(yōu)于同類公司。絕大部分產(chǎn)品是天然成熟型蛋白，而不含有標(biāo)簽蛋白。考慮到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產(chǎn)品沒有添加保護(hù)劑或鹽，這就對工藝提出了更高的要求。同時(shí)，ProSpec-Tany絕大部分產(chǎn)品為凍干粉，因此易于運(yùn)輸和保存。

注意

該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請以廠家的英文說明書為準(zhǔn)。下單前，請瀏覽說明書確認(rèn)。

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