中文名稱

【LLO】重組李斯特菌素-O

英文名字

Recombinant Listeriolysin-O

供應(yīng)商

ProSpec

產(chǎn)品貨號

PRO-320

產(chǎn)品報價

￥詢價/50ug

產(chǎn)品說明書

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購買方式

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產(chǎn)品新聞

背景資料

Listeriolysin O （aka LLO） is a hemolysin produced by Listeria monocytogenes bacteria， the pathogen responsible for causing listeriosis. The toxin may be regarded as a virulence factor， since it is crucial for the virulence of L. monocytogenes. LLO is a single polypeptide protein encoded by the hlyA gene and composed of 529 residues. LLO is a thiol-activated cholesterol-dependent pore forming toxin protein； therefore， it is activated by reducing agents and inhibited by oxidizing agents. Still， LLO differs from other thiol-activated toxins， as its cytolytic activity is maximized at a pH of 5.5. Inside the acidic phagosomes （average pH ~ 5.9） of cells that have phagocytosed L. monocytogenes， LLO is selectively activated by maximizing activity at a pH of 5.5. Following the phagosome lysis by LLO， the bacterium breaks out into the cytosol， where it is able to grow intracellularly， and the toxin has reduced activity in the more basic cytosol. Thus， LLO permits L. monocytogenes to break out from the phagosomes into the cytosol without harming the plasma membrane of the infected cell， which allows the bacteria to live intracellularly， where they are sheltered from extracellular immune system factors such as the complement system and antibodies. LLO also brings about dephosphorylation of histone H3 and deacetylation of histone H4 in the early phases of infection， before entry of L. monocytogenes into the host cell. The pore-forming activity is not implicated in causing the histone modifications. The modifications of the histones affect the down regulation of genes encoding proteins involved in the inflammatory response. Therefore， LLO may be significant in subverting the host immune response to L. monocytogenes. At its NH2-terminus it possesses a 25 residues long typical signal sequence excited during the secretion process. Moreover， in its NH2-terminus there is also a 19 amino acids PEST- like sequence that may target this toxin for degradation. The PEST-like sequence found in LLO and is considered crucial for virulence， given that mutants lacking the sequence lysed the host cell. Nevertheless， contrary to PEST’s supposed role in protein degradation， evidence implies that the PEST-like sequence may control LLO production in the cytosol rather than increase degradation of LLO.

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來源宿主

大腸桿菌中重組表達(dá)

溶解建議

Sterile Filtered clear solution. The protein contains 50mM NaH2PO4， 1mM EDTA， 2.7mM KCl， 1mM DTT， 5% glycerol and 0.5M NaCl.

保存建議

LLO although stable at room temperature for 1 week， should be stored desiccated below -18℃. Please prevent freeze-thaw cycles.

其他

ProSpec 是一家全球著名細(xì)胞因子蛋白生產(chǎn)和研發(fā)的公司，具有20多年的生產(chǎn)經(jīng)驗(yàn)，專注于蛋白（重組及合成）生產(chǎn)研發(fā)。我們的核心產(chǎn)品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長因子、瘦素、干細(xì)胞因子、催乳素、表皮生長因子、胰島素樣生長因子、神經(jīng)生長因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。 領(lǐng)先的生產(chǎn)工藝和規(guī)模使其可以提供毫克到克級蛋白，價格優(yōu)于同類公司。絕大部分產(chǎn)品是天然成熟型蛋白，而不含有標(biāo)簽蛋白。考慮到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產(chǎn)品沒有添加保護(hù)劑或鹽，這就對工藝提出了更高的要求。同時，ProSpec-Tany絕大部分產(chǎn)品為凍干粉，因此易于運(yùn)輸和保存。

注意

該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請以廠家的英文說明書為準(zhǔn)。下單前，請瀏覽說明書確認(rèn)。

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