中文名稱

【U1snRNP-68】重組人U1-snRNP 68kDa蛋白

英文名字

Recombinant Human Small Nuclear Ribonucleoprotein 70kDa

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ProSpec

產(chǎn)品貨號

PRO-445

產(chǎn)品報(bào)價(jià)

￥詢價(jià)/1mg   ￥20ug   ￥5ug

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產(chǎn)品新聞

背景資料

Small nuclear ribonucleoprotein complexes （abbreviated as U-snRNP） are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus and consists of one small uridylate-rich RNA （U1 RNA） complexed with several proteins: the three 68/70 kDa， A， C polypeptides are unique to the U1-snRNP particle， whereas 7 so-called Sm proteins （B/B’， D1， D2， D3， E， F， G） form a core subparticle that is common to all U-snRNP complexes.
Both the U1-specific proteins and the Sm core particle are targets of autoantibodies which classically have been called the RNP and RNP-Sm specificities， respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity and specificity and is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens； also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease （MCTD） and 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells.

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來源宿主

大腸桿菌中重組表達(dá)

溶解建議

Sterile Filtered clear solution. U1-snRNP is supplied in 20mM HEPES buffer pH-7.5， 0.01mM EDTA and 0.02% SDS.

保存建議

Store at 4℃ if entire vial will be used within 2-4 weeks. Store， frozen at -20℃ for longer periods of time. Avoid multiple freeze-thaw cycles.

其他

ProSpec 是一家全球著名細(xì)胞因子蛋白生產(chǎn)和研發(fā)的公司，具有20多年的生產(chǎn)經(jīng)驗(yàn)，專注于蛋白（重組及合成）生產(chǎn)研發(fā)。我們的核心產(chǎn)品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長因子、瘦素、干細(xì)胞因子、催乳素、表皮生長因子、胰島素樣生長因子、神經(jīng)生長因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。 領(lǐng)先的生產(chǎn)工藝和規(guī)模使其可以提供毫克到克級蛋白，價(jià)格優(yōu)于同類公司。絕大部分產(chǎn)品是天然成熟型蛋白，而不含有標(biāo)簽蛋白。考慮到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產(chǎn)品沒有添加保護(hù)劑或鹽，這就對工藝提出了更高的要求。同時(shí)，ProSpec-Tany絕大部分產(chǎn)品為凍干粉，因此易于運(yùn)輸和保存。

注意

該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請以廠家的英文說明書為準(zhǔn)。下單前，請瀏覽說明書確認(rèn)。

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