中文名稱

【肌動(dòng)蛋白】肌動(dòng)蛋白

英文名字

Actin

供應(yīng)商

ProSpec

產(chǎn)品貨號(hào)

PRO-517

產(chǎn)品報(bào)價(jià)

￥詢價(jià)/10ug   ￥1mg   ￥50ug

產(chǎn)品說明書

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購買方式

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產(chǎn)品新聞

背景資料

Actin is a muscle protein localized in the I band of the myofibrils； acting along with myosin， it is responsible for contraction and relaxation of muscle. Each actin protomer binds one molecule of ATP and has one high affinity site for either calcium or magnesium ions， as well as several low affinity sites. Actin exists as a monomer in low salt concentrations， but filaments form rapidly as salt concentration rises， with the consequent hydrolysis of ATP. It occurs in globular （G-actin） and fibrous （F-actin） forms. Actin is found in all eukaryotic cells （except for nematode sperm）. Actin is one of the most highly-conserved proteins， differing by no more than 20% in species as diverse as algae and humans. Its other functions include: cell motility， cell division and cytokinesis， vesicle and organelle movement， cell signaling， and the establishment and maintenance of cell junctions and cell shape.

序列

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來源宿主

兔肌肉組織中提取純化

溶解建議

Sterile Filtered White lyophilized （freeze-dried） powder. The protein was lyophilized from a 1mg/ml solution containing 10mM Tris/HCl buffer pH 8.0， 0.2mM CaCl2， 0.2mM ATP， 1mM DTT and 0.5% （w/v） SDS.It is recommended to reconstitute the lyophilized Actin in sterile 18MΩ-cm H2O not less than 100ug/ml， which can then be further diluted to other aqueous solutions.

保存建議

Lyophilized Actin although stable at room temperature for 3 weeks， should be stored desiccated below -18℃. Upon reconstitution Actin should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.

其他

ProSpec 是一家全球著名細(xì)胞因子蛋白生產(chǎn)和研發(fā)的公司，具有20多年的生產(chǎn)經(jīng)驗(yàn)，專注于蛋白（重組及合成）生產(chǎn)研發(fā)。我們的核心產(chǎn)品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長(zhǎng)因子、瘦素、干細(xì)胞因子、催乳素、表皮生長(zhǎng)因子、胰島素樣生長(zhǎng)因子、神經(jīng)生長(zhǎng)因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。 領(lǐng)先的生產(chǎn)工藝和規(guī)模使其可以提供毫克到克級(jí)蛋白，價(jià)格優(yōu)于同類公司。絕大部分產(chǎn)品是天然成熟型蛋白，而不含有標(biāo)簽蛋白。考慮到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產(chǎn)品沒有添加保護(hù)劑或鹽，這就對(duì)工藝提出了更高的要求。同時(shí)，ProSpec-Tany絕大部分產(chǎn)品為凍干粉，因此易于運(yùn)輸和保存。

注意

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