Desmin is a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.
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來源宿主
大腸桿菌中重組表達(dá)
溶解建議
Sterile Filtered White lyophilized (freeze-dried) powder. Desmin was lyophilized from a 1mg/ml solution containing 30mM Tris-HCI pH 8, 9.5M urea, 2mM DTT, 2mM EDTA and 10mM methylammonium chloride. It is recommended to reconstitute the lyophilized Desmin in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
保存建議
Lyophilized Desmin although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution Desmin should be stored at 4℃ between 2-7 days and for future use below -18℃.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.