中文名稱

【OmpA】重組細(xì)菌外膜蛋白-A

英文名字

Recombinant Bacterial Outer Membrane Protein-A

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ProSpec

產(chǎn)品貨號(hào)

PRO-571

產(chǎn)品報(bào)價(jià)

￥詢價(jià)/200ug

產(chǎn)品說明書

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產(chǎn)品新聞

背景資料

The OmpA protein is one of the main outer-membrane proteins of a large array of Gram-negative bacteria such as A. salmonicida， Shigella dysenteriae and E. coli. OmpA’s major physiological functions include maintenance of the structural integrity and morphology of the cells and porin activity， as well as a role in conjugation and bacteriophage binding. Achromogenic atypical Aeromonas salmonicida is the causative agent of goldfish ulcer disease. Virulence of this bacterium is associated with the production of a paracrystalline outer membrane A-layer protein. The species specific structural gene for the monomeric form of A-protein was cloned into a pET-3d plasmid in order to express and produce a recombinant form of the protein in E. coli BL21（DE3）. The induced protein was isolated from inclusion bodies by a simple solubilization-renaturation procedure and purified by ion exchange chromatography on Q-Sepharose to over 95% pure monomeric protein. Recombinant A-protein was compared by biochemical， immunological and molecular methods with the A-protein isolated from atypical A. salmonicida bacterial cells by the glycine and the membrane extraction methods.

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來源宿主

大腸桿菌中重組表達(dá)

溶解建議

Sterile Filtered White lyophilized （freeze-dried） powder. It is recommended to reconstitute the lyophilized OmpA in sterile 0.4% NaHCO3.

保存建議

Lyophilized Bacterial Outer Membrane Protein-A although stable at room temperature for 3 weeks， should be stored desiccated below -18℃. Upon C between 2-7 days and for future usereconstitution OmpA should be stored at 4 below -18℃. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.

其他

ProSpec 是一家全球著名細(xì)胞因子蛋白生產(chǎn)和研發(fā)的公司，具有20多年的生產(chǎn)經(jīng)驗(yàn)，專注于蛋白（重組及合成）生產(chǎn)研發(fā)。我們的核心產(chǎn)品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長因子、瘦素、干細(xì)胞因子、催乳素、表皮生長因子、胰島素樣生長因子、神經(jīng)生長因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。 領(lǐng)先的生產(chǎn)工藝和規(guī)模使其可以提供毫克到克級(jí)蛋白，價(jià)格優(yōu)于同類公司。絕大部分產(chǎn)品是天然成熟型蛋白，而不含有標(biāo)簽蛋白。考慮到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產(chǎn)品沒有添加保護(hù)劑或鹽，這就對(duì)工藝提出了更高的要求。同時(shí)，ProSpec-Tany絕大部分產(chǎn)品為凍干粉，因此易于運(yùn)輸和保存。

注意

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