【SPARC】重組人殘余半胱氨酸酸性分泌蛋白-Recombinant Human Secreted Protein Acidic & Rich in Cysteine, His Tag代理/參數(shù)/廠家-蛋白標(biāo)準(zhǔn)品-艾美捷科技有限公司

PRODUCT CENTER

蛋白標(biāo)準(zhǔn)品

產(chǎn)品中心

購(gòu)買方式: 在線支付寶及網(wǎng)銀支付、支票、現(xiàn)金、電匯，或撥打全國(guó)免費(fèi)服務(wù)電話4006800868與我們聯(lián)系.

背景資料: SPARC， an acronym for “secreted protein， acidic and rich in cysteine”， is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid， 43 kDa protein contains a 17 aa signal sequence， an N-terminal acidic region that binds calcium， a follistatin domain containing Kazal-like sequences， and a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts， capillary endothelial cells， platelets and macrophages， especially in areas of tissue morphogenesis and remodeling. SPARC shows context-specific effects， but generally inhibits adhesion， spreading and proliferation， and promotes collagen matrix formation. For endothelial cells， SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF. SPARC is abundantly expressed in bone， where it promotes osteoblast differentiation and inhibits adipogenesis.

溶解建議: Sterile Filtered White lyophilized （freeze-dried） powder. The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 ug/ml， which can then be further diluted to other aqueous solutions.

保存建議: Lyophilized Osteonectin although stable at room temperature for 3 weeks， should be stored desiccated below -18℃. Upon reconstitution BM-40 should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.

其他: ProSpec 是一家全球著名細(xì)胞因子蛋白生產(chǎn)和研發(fā)的公司，具有20多年的生產(chǎn)經(jīng)驗(yàn)，專注于蛋白（重組及合成）生產(chǎn)研發(fā)。我們的核心產(chǎn)品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長(zhǎng)因子、瘦素、干細(xì)胞因子、催乳素、表皮生長(zhǎng)因子、胰島素樣生長(zhǎng)因子、神經(jīng)生長(zhǎng)因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。領(lǐng)先的生產(chǎn)工藝和規(guī)模使其可以提供毫克到克級(jí)蛋白，價(jià)格優(yōu)于同類公司。絕大部分產(chǎn)品是天然成熟型蛋白，而不含有標(biāo)簽蛋白。考慮到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產(chǎn)品沒(méi)有添加保護(hù)劑或鹽，這就對(duì)工藝提出了更高的要求。同時(shí)，ProSpec-Tany絕大部分產(chǎn)品為凍干粉，因此易于運(yùn)輸和保存。