TEV protease is the common name for the 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the tobacco etch virus (TEV). Because its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokinase, TEV protease is a very useful reagent for cleaving fusion proteins. TEV protease recognizes a linear epitope of the general form E-Xaa-Xaa-Y -Xaa-Q-(G/S), with cleavage occurring between Q and G or Q and S. The most commonly used sequence is ENLYFQG.
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來源宿主
大腸桿菌中重組表達
溶解建議
Sterile liquid formulation. The rTEV contains 0.50 M Tris-HCl pH 8.0, 10mM DTT and 5 mM EDTA.
保存建議
Store rTEV at -70?C for long term or at -20?C for< 6 months. Please prevent freeze-thaw cycles.