中文名稱

【AMBP】重組人alpha-1微球蛋白

英文名字

Recombinant Human Alpha-1 Microglobulin

供應(yīng)商

ProSpec

產(chǎn)品貨號(hào)

PRO-957

產(chǎn)品報(bào)價(jià)

￥詢價(jià)/10ug   ￥1mg   ￥2ug

產(chǎn)品說明書

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購買方式

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產(chǎn)品新聞

背景資料

Alpha 1-microglobulin （A1M） is a lipocalin superfamily member （kernal lipocalins）. A1M is a low molecular weight protein component of plasma. A1M is distributed in plasma and extravascular compartments of all organs. Alpha-1 Microglobulin is found in mammals， birds， amphibians and fish. The primary sites of A1M synthesis are the liver and the kidney. Around the opening of the lipocalin pocket three lysyl residues are situated； those residues carry yellow-brown modification derived from the binding and degradation of heme and kynurenin （a tryptophan metabolite）. A1-Microglobulin’s reductase and dehydrogenase have broad biological substrate specificity properties due to its’ free cysteine side-chain which is located in a flexible loop. Alpha-1-microglobulin is glycosylated by three separate carbohydrate chains: two complex carbohydrates which are N-linked to asparagines at residues 17 and 96， and the other simple carbohydrate which is O-linked to threonine at position 5. The carbohydrates comprise 22% of the total molecular mass of the protein. The glycosylation varies between species.
A1M exists in two forms- a free form and complexed to other macromolecules: in humans- complexed to immunoglobulin A （IgA）， in rat- complexed to alpha-1-inhibitor-3. Free A1M is exceptionally heterogeneous in charge （therefore also known as protein HC）， and is found tightly linked to a chromophore. The free Alpha-1-microglobulin is a monomeric protein composed of one 188 residue polypeptide and contains three cysteines， two of which （residues 75 and 173） form a conserved intra-molecular disulphide link. The chromophoric group is covalently bound to the free cysteine residue at position 34. A1M binds retinol as a major ligand， but this is probably distinct from its covalent chromophore.
Half of all human plasma A1M （approximately 0.03mg/ml） forms a 1:1 complex with about 5% of plasma immunoglobulin A. The resulting macromolecular complexes’ molecular weight is 200000， and a plasma concentration of 0.3mg/ml. The complex can exhibit both antibody activity and affect many of the biological actions of free Alpha-1-microglobulin.
Alpha-1-microglobulin was first discovered in pathological human urine.
It was suggested that A1M might be involved in tissue defense against reactive oxygen species， oxidation by heme and kynurenin. Evidence also suggests that A1M functions in the regulation of the immune system. Other functions include: inhibition of stimulation of cultured lymphocytes by protein antigens； induction of cell division of lymphocytes， a mitogenic effect that can either be enhanced or inhibited by the action of other plasma components； inhibition of neutrophil granulocyte migration in vitro； and inhibition of chemotaxis.

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來源宿主

大腸桿菌中重組表達(dá)

溶解建議

提供的重組人alpha-1微球蛋白產(chǎn)品形式為經(jīng)無菌過濾處理的無色溶液.

保存建議

重組人alpha-1微球蛋白產(chǎn)品在4℃保存請2 - 4周內(nèi)將使用；-20℃可長期保存.產(chǎn)品若長期保存，建議添加載體蛋白（0.1% HSA或BSA）.避免反復(fù)凍融.

其他

ProSpec 是一家全球著名細(xì)胞因子蛋白生產(chǎn)和研發(fā)的公司，具有20多年的生產(chǎn)經(jīng)驗(yàn)，專注于蛋白（重組及合成）生產(chǎn)研發(fā)。我們的核心產(chǎn)品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長因子、瘦素、干細(xì)胞因子、催乳素、表皮生長因子、胰島素樣生長因子、神經(jīng)生長因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。 領(lǐng)先的生產(chǎn)工藝和規(guī)模使其可以提供毫克到克級(jí)蛋白，價(jià)格優(yōu)于同類公司。絕大部分產(chǎn)品是天然成熟型蛋白，而不含有標(biāo)簽蛋白。考慮到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產(chǎn)品沒有添加保護(hù)劑或鹽，這就對工藝提出了更高的要求。同時(shí)，ProSpec-Tany絕大部分產(chǎn)品為凍干粉，因此易于運(yùn)輸和保存。

注意

該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請以廠家的英文說明書為準(zhǔn)。下單前，請瀏覽說明書確認(rèn)。

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