WB; IHC, Frozen; IF, Frozen; ELISA; Neut, Neutralizing IHC, ELISA (1 site), Western Blot, immunoblot, inhibition of biological activity in vitro/in vivo. Recommended to be used at an amount of 1-10 μg/mL for immunohistochemistry, Western blot or immunoblot, 0.1 μg/mL for ELISA and for inhibition of biological activity in vitro 1-10 μg/mL. Use neat for in vivo studies at 2-10 μg/mL (ED50). This antiserum stains cell bodies and some nerve terminals in the dorsal horn of the rat spinal cord, however, does not stain finest nerve terminals. Western blot: 1-10 μg/mL. Tissue homogenate is a recommended sample application for Western Blotting. The antibody detects 14 kDa (mature BDNF), 32 kDa (proBDNF) and a 18 kDa BDNF isoform, however numerous other non-characterized bands may also be present. In cell lysates, only 18 kDa and 32 kDa BDNF are detected. Alternative antibodies for Western Blotting are: R-1707-100 (cell lysates and tissue homogenates), R-083-100/R-066-500 (cell lysates, tissue homogenates and human serum); M-1744-50/100 (human serum and tissue homogenates).
Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
Recombinant human BDNF
來(lái)源宿主
兔
反應(yīng)性
This antiserum is known to react with BDNF from 大鼠, 小鼠, 人. Expected to react with BDNF of other species due to amino acid sequence homology.