WB; IHC, Frozen; ELISA Western Blotting: A concentration of 1-10 μg/mL is recommended for this application. In Western Blotting, this antibody detects multiple BDNF isoforms (14 kDa mature BDNF, 18 kDa isoform, 28 kDa BDNF dimer/truncated BDNF, 32 kDa proBDNF monomer) depending on sample application (human serum, cell lysate, tissue homogenate).
IHC: Antibody works well in immunohistochemistry with the proper fixation, pretreatments and dilution. Formal fixed, paraffin embedded tissue is not recommend. Recommended fixation is Zamboni fixative or light 4% PFA fixation on fixed, frozen tissue. Recommended dilution is 1-10 μg/mL for immunohistochemistry at 4 degrees centigrade for 2-48 hours. ELISA: 1-10 μg/mL capture/detection.
Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst mammalian species.
來源宿主
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反應(yīng)性
This antiserum is known to recognise 大鼠, 人, 人 BDNF,, is expected to react with BDNF from other species due to amino acid sequence homology.