WB; IHC, Frozen; IF, Frozen; ELISA,Neut, Neutralizing IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 μg/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 μg/mL (ED50). This antibody was tested on cultured sensory neurons supported by 100 ng/mL of purified mouse beta NGF. Be advised that 2 μg/mL will neutralize 100 ng/mL of mouse NGF. The higher 10 μg/mL is only recommended if the concentration of NGF being used is higher than 100 ng/mL such as the 200 or 500ng/mL that is occasionally used in some culture systems. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
來源宿主
兔
反應性
This antiserum is known to cross react with 小鼠, 大鼠, 人, avian NGF but not 牛 NGF.