IH,Frozen, IC Immunocytochemistry (ICC): 1:2,000-1:10,000.
Immunohistochemistry (IHC): 1:20,000-1:50,000. This antibody has been shown to work on 4% PFA fixed mouse brain sections. Note that non-specific staining has been observed on tissue sections when using this antibody at dilutions of 1:5,000 or lower. Click here for instructions on use of this antibody in IHC on free-floating brain sections.
Western blotting (WB): 1:1,000-1:2,000. This antibody detects bands between 50-65 kDa, which only appear in stimulated cells.
Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
Full length, E.coli-derived recombinant human c-FOS protein.