RBP4, Human, BioAssay ELISA Kit (Retinol-binding Protein 4, Plasma Retinol-binding Protein, PRBP, RBP, PRO2222)-RBP4, Human, BioAssay ELISA Kit (Retinol-binding Protein 4, Plasma Retinol-binding Protein, PRBP, RBP, PRO2222)代理/參數(shù)/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: RBP4, Human, BioAssay ELISA Kit (Retinol-binding Protein 4, Plasma Retinol-binding Protein, PRBP, RBP, PRO2222)

英文名字: RBP4, Human, BioAssay ELISA Kit (Retinol-binding Protein 4, Plasma Retinol-binding Protein, PRBP, RBP, PRO2222)

供應商: USBiological

產品貨號: R1701-36V

產品報價: ￥1/100Tests

產品說明書: 點擊查看

購買方式: 銀行轉賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868

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產品描述: Retinol binding protein (RBP) 4 is the only specific transport protein for vitamin A in the circulation whose function is to deliver vitamin to target tissues. In obesity and type 2 diabetes, expression of Glut4 is significantly impaired in adipocytes. Glucose transport via Glut4 is the rate-limiting step for glucose use by muscle and adipose tissue. Adipocyte-specific deletion of Gluts leads to notable elevation of RBP4 causing systemic insulin resistance, and that reduction of RBP4 improves insulin resistance. This identified a novel role of RBP4 in regulating insulin action and RBP4 is recorded as an adipocyte-derived hormone. The RBP4 (human) ELISA Kit is to be used for the in vitro quantitative determination of human RBP4 in biological fluids. This assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG, peroxidase and TMB (3,3',5,5'-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of RBP4 in the samples. This ELISA is specific for the measurement of natural and recombinant human RBP4. It does not cross-react with mouse RBP4, rat RBP4, human adiponectin, human resistin, human vaspin, human clusterin, human leptin, human IL-33, human GPX3, human progranulin, human FABP4, human ANGPTL3, human ANGPTL4, human ANG1, human ANG2, human Nampt, mouse Nampt. The assay range is 0.39-25ng RBP4/ml and a detection limit of 380pg/ml (based on adding 2 standard deviations to the mean value of the (50) zero standards).Sample Type:Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids.Intended Use:For the in vitro quantitative determination of human RBP4 in biological fluids.Sensitivity:380pg/mlRange:0.39-25ng RBP4/mlSpecificity:MammalianTest Principle:This assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG, peroxidase and TMB (3,3',5,5'-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of RBP4 in the samples. This ELISA is specific for the measurement of natural and recombinant human RBP4. It does not cross-react with mouse RBP4, rat RBP4, human adiponectin, human resistin, human vaspin, human clusterin, human leptin, human IL-33, human GPX3, human progranulin, human FABP4, human ANGPTL3, human ANGPTL4, human ANG1, human ANG2, human Nampt, mouse Nampt.Kit Components:Pre-coated Microtiter Plate: 12x8 well stripsWash Buffer (10X): 1x50mlDiluent (5X):1x50mlDetection Antibody: 1x12mlDetector 100X (Hrp conjugated anti-IgG): 1x150ulHuman RBP4 Standard (lyophilized, 50ng): 1 vialHuman RBP4 QC Sample (lyophilized): 1 vialSubstrate Solution I (TMB): 1x6mlSubstrate Solution II (Peroxidase): 1x6mlStop Solution: 1x12mlPlate Sealers: 3 eachStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。