The United States Biological Rubella, Human, IgG, BioAssay ELISA Kit is intended for use in evaluating a sample's serologic status to rubella virus infection. It is also used to evaluate paired sera for the presence of a significant increase in specific IgG as indicative of a recent or current rubella virus infection.Rubella is a herpes virus. Generally rubella is considered a mild adolescence disease. However a maternal infection could be transmitted through the placenta to the fetus, causing congenital rubella. Congenital rubella may result in chronic cardiac disease, growth retardation, hepatosplenomegaly, malformations and other severe anomalies. Children born asymptomatic may develop these abnormalities later in life. To reduce risk of such severe complications, accurate serological methods must be performed to determine the serologic status of childbearing aged women. The presence of rubella specific IgG in the bloodstream attests immunity to rubella.Kit Components:1. Microtiter Plate: 1x96 wells2. Enzyme conjugate: 1X12 ml3. Negative Calibrator: 1X150ul4. Cut-off Calibrator: 1X150ul5. Positive Calibrator: 2X150ul 6. Negative control: 1X150ul7. Positive Control: 1X150ul8. Wash Buffer,10X: 1X100ml9. Sample diluent: 1X22ml 10. Tetramethylbenzidine (TMB): 1x15ml 11. Stop solution:1X12 ml (2 N Hcl)Storage and Stability:Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.