WB; IHC, Frozen; ELISA; Neut, Neutralizing IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A dilution of 1:1000-1:5000 is recommended for IHC, western blot and immunoblot; 1:15000 for ELISA; for inhibition of biological activity: 1:10-50 for in vitro, 5-10 μL/g body weight for in vivo. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
來源宿主
綿羊
反應(yīng)性
This antiserum is known to cross react with 小鼠, 大鼠, 人, avian NGF but not 牛 NGF.