S6 Ribosomal Protein, Total, BioAssay ELISA Kit discontinued-S6 Ribosomal Protein, Total, BioAssay ELISA Kit discontinued代理/參數(shù)/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: S6 Ribosomal Protein, Total, BioAssay ELISA Kit discontinued

英文名字: S6 Ribosomal Protein, Total, BioAssay ELISA Kit discontinued

供應商: USBiological

產(chǎn)品貨號: S0050-45

產(chǎn)品報價: ￥1/1Kit

產(chǎn)品說明書: 點擊查看

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背景資料: USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業(yè)銷售團隊、技術(shù)支持團隊、市場營銷團隊、進出口報關(guān)團隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務。我們致力于為您提供有價值的產(chǎn)品和服務，在意您的成功！

產(chǎn)品描述: To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylates the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions (2). This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors (2,3). The main in vivo S6 ribosomal protein phosphorylation sites, including Ser235, Ser236, Ser240 and Ser244, are located within a small 19 amino acid region in the S6 carboxy terminus (4,5).Total S6 Ribosomal Protein Sandwich ELISA Antibody Pair is being offered as an alternative to our Total S6 Ribosomal Protein Sandwich ELISA Kit. Capture and Detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are provided for performing 4 x 96 well ELISAs. The S6 Ribosomal Protein Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added, followed by S6 Ribosomal Protein Detection Antibody and HRP-conjugated secondary antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance at 450 nm is proportional to the quantity of Total S6 Ribosomal protein.Kit Components: S6 Ribosomal Protein Capture Antibody (100X), 1x0.4ml S6 Ribosomal Protein Detection Antibody (100X), 1x0.4ml Anti-Rabbit IgG HRP-Linked Antibody (1000X), 1x0.04ml Reagents not supplied: Phosphate Buffered Saline (PBS-20X)Phosphate Buffered Saline with Tween -20 (PBST-20X) Cell Lysis Buffer (10X)TMB SubstrateSTOP SolutionBlocking Buffer- PBS+0.05% Tween-20, 1% BSA 96 Well MicroplatesMicroplate Reader Storage and Stability:S6 Ribosomal Protein Capture Antibody and S6 Ribosomal Protein Detection Antibody are stored at 4°C. Anti-Rabbit IgG-HRP Linked Antibody is stored at -20°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.?

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保存建議: 建議收到S6 Ribosomal Protein, Total, BioAssay ELISA Kit discontinued產(chǎn)品后將其置于-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。