- 中文名稱
SIRT2, Human, BioAssay ELISA Kit (NAD-dependent Protein Deacetylase Sirtuin-2, Regulatory Protein SIR2 Homolog 2, SIR2-like Protein 2, SIR2L, SIR2L2)
- 英文名字
- SIRT2, Human, BioAssay ELISA Kit (NAD-dependent Protein Deacetylase Sirtuin-2, Regulatory Protein SIR2 Homolog 2, SIR2-like Protein 2, SIR2L, SIR2L2)
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號(hào)
- S1113-82
- 產(chǎn)品報(bào)價(jià)
- ￥1/100Tests
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場營銷團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！
- 產(chǎn)品描述
- Sirtuin 2 is a NAD-dependent histone deacetylase (39.5kD) largely localized at the cytoplasm where it is physically associated with microtubules. SIRT2 was found to deacetylate lys40 of alpha-tubulin both in vitro and in vivo and colocalized and interacted in vivo with HDAC6, another tubulin deacetylase. It has been also shown that Sirtuin 2 is a regulator of mitotic progression that acts downstream from CDC14B in a pathway regulating mitotic exit or subsequent cytokinesis. This assay is a sandwich ELISA for quantitative determination of human Sirtuin 2 in cells. A monoclonal antibody specific for Sirtuin 2 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, Sirtuin 2 is recognized by the addition of a purified polyclonal antibody specific for Sirtuin 2 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of Sirtuin 2 in the samples. This ELISA is specific for the measurement of natural and recombinant human Sirtuin 2. It does not cross-react with human Sirtuin 1, human Sirtuin 5, human Sirtuin 6, human adiponectin, human resistin, human RBP4, human vaspin, human progranulin, human GPX3, human FTO, human Nampt, human leptin, mouse FTO, mouse Nampt. The assay range is 0.125-8ng Sirtuin 2/ml and a detection limit of 80pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).Sample Type:Cell lysates.Intended Use:For the quantitative determination of human Sirtuin 2 in cells.Sensitivity:80pg/ml Range:0.125-8ng Sirtuin 2/mlSpecificity:MammalianTest Principle:This assay is a sandwich ELISA for quantitative determination of human Sirtuin 2 in cells. A monoclonal antibody specific for Sirtuin 2 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, Sirtuin 2 is recognized by the addition of a purified polyclonal antibody specific for Sirtuin 2 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of Sirtuin 2 in the samples.Kit Components:Pre-coated Microtiter Plate: 12x8 well stripsWash Buffer (10X): 1x50mlDiluent (5X):1x50mlLysis Buffer (10X): 1x12mlDetection Antibody: 1x12mlDetector 100X (HRP conjugated anti-IgG): 1x150ulHuman Sirtuin 2 Standard (lyophilized): 16ngHuman Sirtuin 2 QC Sample (lyophilized): 1 vialSubstrate Solution (TMB): 1x12mlStop Solution: 1x12mlPlate Sealers: 3 eachStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產(chǎn)品特點(diǎn)
- 針對(duì)SIRT2, Human, BioAssay ELISA Kit (NAD-dependent Protein Deacetylase Sirtuin-2, Regulatory Protein SIR2 Homolog 2, SIR2-like Protein 2, SIR2L, SIR2L2)該產(chǎn)品的特點(diǎn)優(yōu)勢，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
- 保存建議
- 建議收到SIRT2, Human, BioAssay ELISA Kit (NAD-dependent Protein Deacetylase Sirtuin-2, Regulatory Protein SIR2 Homolog 2, SIR2-like Protein 2, SIR2L, SIR2L2)產(chǎn)品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說明書為準(zhǔn)。下單前，請(qǐng)瀏覽說明書確認(rèn)。
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