Oxidative Stress/ Damage->Reactive Oxygen Species (ROS) Assays->Intracellular ROS Assay
應(yīng)用類型
The OxiSelect? ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on?a standard fluorometric plate reader.