Cell Signaling and Protein Biology->Kinase Assays->Rho Kinase (ROCK) Assays
應(yīng)用類型
Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase?in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.
免疫原
Safe non-radioactive ELISA format
來源宿主
(96-Well ROCK Activity Assay Kit)保存建議,請查看該產(chǎn)品的使用手冊